FastQCFastQC Report
Fri 16 Dec 2022
EGAF00007543415

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007543415
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences88996323
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGAGCTCAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGACT34889743.9203574736452875TruSeq Adapter, Index 4 (97% over 37bp)
ACAACCGGTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGACT32206993.618912435292411TruSeq Adapter, Index 4 (97% over 37bp)
CAAGAGCTCTTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGACT19880212.233823750223928TruSeq Adapter, Index 4 (97% over 37bp)
TGCTGCGCAGCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGACT10942851.2295845076655583TruSeq Adapter, Index 4 (97% over 37bp)
CTATTGCAATAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGACT10366651.164840259748709TruSeq Adapter, Index 4 (97% over 37bp)
CCATAGCTATGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGACT9378441.0538008407381056TruSeq Adapter, Index 4 (97% over 37bp)
GCATTCGAATGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGACT8474510.9522314759004145TruSeq Adapter, Index 4 (97% over 37bp)
TATCTCGAGATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGACT5909790.664048783228943TruSeq Adapter, Index 4 (97% over 37bp)
GTGAGGCCTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGACT5483190.6161142185615915TruSeq Adapter, Index 4 (97% over 37bp)
ATCGACGTCGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGACT3817330.42893120427009107TruSeq Adapter, Index 4 (97% over 37bp)
CTCAGCGCTGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGACT3689850.4146070169663077TruSeq Adapter, Index 4 (97% over 37bp)
CATGACGTCATGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGACT3627850.407640437009965TruSeq Adapter, Index 4 (97% over 37bp)
AGGATGCATCCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGACT2405490.27029094224488354TruSeq Adapter, Index 4 (97% over 37bp)
ACTCACGTGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGACT2314470.2600635534122011TruSeq Adapter, Index 4 (97% over 37bp)
TGTAGGCCTACAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGACT2274760.2556015713143564TruSeq Adapter, Index 4 (97% over 37bp)
GTTGAGCTCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGACT1789660.20109370136561708TruSeq Adapter, Index 4 (97% over 37bp)
GAAGTCGACTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGACT1666960.18730661490362924TruSeq Adapter, Index 4 (97% over 37bp)
GTTGAGCTTAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGACT1544760.17357571053806348TruSeq Adapter, Index 4 (97% over 37bp)
TCTCTGCAGAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGACT1480340.1663372092350377TruSeq Adapter, Index 4 (97% over 37bp)
AGGATGTTGTAGAGTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1238380.13914956913444615No Hit
ATAACCGGTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGACT1184630.1331099937690684TruSeq Adapter, Index 4 (97% over 37bp)
TGACAGCTGTCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGACT1164750.130876193615325TruSeq Adapter, Index 4 (97% over 37bp)
CGGTAGCTACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGACT1136800.12773561442532858TruSeq Adapter, Index 4 (97% over 37bp)
AGAAGGCCTTCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGACT938940.10550323522916785TruSeq Adapter, Index 4 (97% over 37bp)
TAAGAGCTCTTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGACT895460.10061764012430043TruSeq Adapter, Index 4 (97% over 37bp)
AGGATGTTGTAGAGTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA890210.10002772811186816No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)