FastQCFastQC Report
Fri 16 Dec 2022
EGAF00007543416

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007543416
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences88996323
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGAGCTCAACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT36994834.156894212359762Illumina Single End PCR Primer 1 (100% over 39bp)
ACAACCGGTTGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT33040713.7125927101505076Illumina Single End PCR Primer 1 (100% over 39bp)
CAAGAGCTCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT20211932.2710972002742182Illumina Single End PCR Primer 1 (100% over 39bp)
TGCTGCGCAGCAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT11777451.3233636630133585Illumina Single End PCR Primer 1 (100% over 39bp)
CTATTGCAATAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT10517701.181812871077831Illumina Single End PCR Primer 1 (100% over 39bp)
CCATAGCTATGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT9373891.0532895836606642Illumina Single End PCR Primer 1 (100% over 39bp)
GCATTCGAATGCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT8609610.9674118783536708Illumina Single End PCR Primer 1 (100% over 39bp)
TATCTCGAGATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT6060070.680934874129575Illumina Single End PCR Primer 1 (100% over 39bp)
GTGAGGCCTCACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT5887620.6615576690735864Illumina Single End PCR Primer 1 (100% over 39bp)
CTCAGCGCTGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT3931890.4418036462023268Illumina Single End PCR Primer 1 (100% over 39bp)
ATCGACGTCGATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT3909230.43925747359247636Illumina Single End PCR Primer 1 (100% over 39bp)
CATGACGTCATGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT3677900.4132642648618191Illumina Single End PCR Primer 1 (100% over 39bp)
AGGATGCATCCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT2453080.2756383541823408Illumina Single End PCR Primer 1 (100% over 39bp)
TGTAGGCCTACAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT2406730.27043027384401036Illumina Single End PCR Primer 1 (100% over 39bp)
ACTCACGTGAGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT2329080.2617051942696554Illumina Single End PCR Primer 1 (100% over 39bp)
ATTGAGCTCAACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT1819660.20446462715094418Illumina Single End PCR Primer 1 (100% over 39bp)
GAAGTCGACTTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT1729000.19427768942768567Illumina Single End PCR Primer 1 (100% over 39bp)
GTTAAGCTCAACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT1585510.17815455139646613Illumina Single End PCR Primer 1 (100% over 39bp)
TCTCTGCAGAGAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT1489560.16737320709306158Illumina Single End PCR Primer 1 (100% over 39bp)
TGACAGCTGTCAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT1251190.1405889544447808Illumina Single End PCR Primer 1 (100% over 39bp)
ACAACCGGTTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT1211180.1360932630890829Illumina Single End PCR Primer 1 (100% over 39bp)
CGGTAGCTACCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT1107050.12439277968821251Illumina Single End PCR Primer 1 (100% over 39bp)
ACAACCGGTTGTAAATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT1099760.12357364472237803Illumina Single End PCR Primer 1 (97% over 39bp)
AGAAGGCCTTCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT962910.1081966049316442Illumina Single End PCR Primer 1 (100% over 39bp)
CAAGAGCTCTTAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT921550.10354922191560656Illumina Single End PCR Primer 1 (100% over 39bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)