FastQCFastQC Report
Fri 16 Dec 2022
EGAF00007543417

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007543417
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81379196
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAACCGGTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGGC41251215.069011741035141TruSeq Adapter, Index 7 (97% over 36bp)
GTTGAGCTCAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGGC29429093.616291564246961TruSeq Adapter, Index 7 (97% over 36bp)
TGCTGCGCAGCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGGC20401972.5070252598710856TruSeq Adapter, Index 7 (97% over 36bp)
CAAGAGCTCTTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGGC8431251.0360448879342576TruSeq Adapter, Index 7 (97% over 36bp)
CCATAGCTATGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGGC8386931.0305987785870974TruSeq Adapter, Index 7 (97% over 36bp)
CTATTGCAATAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGGC7003450.8605946414117928TruSeq Adapter, Index 7 (97% over 36bp)
TATCTCGAGATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGGC6735120.8276218408449255TruSeq Adapter, Index 7 (97% over 36bp)
GCATTCGAATGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGGC2842810.34932883829424904TruSeq Adapter, Index 7 (97% over 36bp)
GTGAGGCCTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGGC2366100.29074998479955494TruSeq Adapter, Index 7 (97% over 36bp)
TGTAGGCCTACAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGGC2166770.266256009705478TruSeq Adapter, Index 7 (97% over 36bp)
AGGATGCATCCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGGC1960240.24087728760554478TruSeq Adapter, Index 7 (97% over 36bp)
CATGACGTCATGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGGC1894010.23273884396695196TruSeq Adapter, Index 7 (97% over 36bp)
ACTCACGTGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGGC1779620.2186824259114086TruSeq Adapter, Index 7 (97% over 36bp)
CTCAGCGCTGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGGC1733510.21301635862807003TruSeq Adapter, Index 7 (97% over 36bp)
AGAAGGCCTTCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGGC1475300.18128711913054538TruSeq Adapter, Index 7 (97% over 36bp)
ATCGACGTCGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGGC1367740.16806998191527966TruSeq Adapter, Index 7 (97% over 36bp)
GTTGAGCTCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGGC1319210.16210654133275046TruSeq Adapter, Index 7 (97% over 36bp)
GTTGAGCTTAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGGC1243940.15285724867569347TruSeq Adapter, Index 7 (97% over 36bp)
GAAGTCGACTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGGC1204260.14798130962119607TruSeq Adapter, Index 7 (97% over 36bp)
TGACAGCTGTCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGGC1188580.1460545272528866TruSeq Adapter, Index 7 (97% over 36bp)
ATAACCGGTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGGC1101740.13538349531002986TruSeq Adapter, Index 7 (97% over 36bp)
TCTCTGCAGAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGGC1035620.12725856863958204TruSeq Adapter, Index 7 (97% over 36bp)
ACAATCGGTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGGC967500.11888787891195188TruSeq Adapter, Index 7 (97% over 36bp)
TGTTGCGCAGCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGGC923680.11350321032908707TruSeq Adapter, Index 7 (97% over 36bp)
TGCTGCGCAGTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGGC840260.10325243321401209TruSeq Adapter, Index 7 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)