FastQCFastQC Report
Thu 2 May 2024
EGAF00007543418

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007543418
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81379196
Total Bases4.1 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAACCGGTTGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC41874765.145634518188162Illumina Single End PCR Primer 1 (100% over 38bp)
GTTGAGCTCAACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC30428583.739110423258544Illumina Single End PCR Primer 1 (100% over 38bp)
TGCTGCGCAGCAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC21421342.632287003671061Illumina Single End PCR Primer 1 (100% over 38bp)
CAAGAGCTCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC8471271.040962606708476Illumina Single End PCR Primer 1 (100% over 38bp)
CCATAGCTATGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC8280511.0175217263144256Illumina Single End PCR Primer 1 (100% over 38bp)
CTATTGCAATAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC7073110.8691545686934534Illumina Single End PCR Primer 1 (100% over 38bp)
TATCTCGAGATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC6866200.8437291516126554Illumina Single End PCR Primer 1 (100% over 38bp)
GCATTCGAATGCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC2841400.34915557533893554Illumina Single End PCR Primer 1 (100% over 38bp)
GTGAGGCCTCACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC2469070.30340309579858715Illumina Single End PCR Primer 1 (100% over 38bp)
TGTAGGCCTACAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC2243740.27571420096114Illumina Single End PCR Primer 1 (100% over 38bp)
AGGATGCATCCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC1982620.24362737621541505Illumina Single End PCR Primer 1 (100% over 38bp)
CATGACGTCATGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC1907770.2344296938003664Illumina Single End PCR Primer 1 (100% over 38bp)
CTCAGCGCTGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC1814280.2229414996923784Illumina Single End PCR Primer 1 (100% over 38bp)
ACTCACGTGAGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC1785740.21943446086638654Illumina Single End PCR Primer 1 (100% over 38bp)
AGAAGGCCTTCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC1493330.18350267309104404Illumina Single End PCR Primer 1 (100% over 38bp)
ATCGACGTCGATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC1386840.17041701911137092Illumina Single End PCR Primer 1 (100% over 38bp)
ATTGAGCTCAACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC1327160.1630834494850502Illumina Single End PCR Primer 1 (100% over 38bp)
GTTAAGCTCAACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC1259950.15482458194843804Illumina Single End PCR Primer 1 (100% over 38bp)
TGACAGCTGTCAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC1235070.15176728951708984Illumina Single End PCR Primer 1 (100% over 38bp)
GAAGTCGACTTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC1228340.1509402968296713Illumina Single End PCR Primer 1 (100% over 38bp)
ACAACCGGTTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC1123980.13811638050589736Illumina Single End PCR Primer 1 (100% over 38bp)
TCTCTGCAGAGAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC1036630.12738267898345912Illumina Single End PCR Primer 1 (100% over 38bp)
ACAACCGATTGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC969800.11917050642771157Illumina Single End PCR Primer 1 (100% over 38bp)
TGCTGCGCAACAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC935700.11498024630275286Illumina Single End PCR Primer 1 (100% over 38bp)
TACTGCGCAGCAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC851610.10464713856352181Illumina Single End PCR Primer 1 (100% over 38bp)
ACAACCGGTTGTAAATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC850550.10451688414321518Illumina Single End PCR Primer 1 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)