FastQCFastQC Report
Fri 16 Dec 2022
EGAF00007543419

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007543419
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences94469906
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAACCGGTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCTC23342492.470891629764086TruSeq Adapter, Index 1 (97% over 36bp)
CCATAGCTATGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCTC6946960.7353622221239429TruSeq Adapter, Index 1 (97% over 36bp)
CAAGAGCTCTTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCTC6285470.6653409817090323TruSeq Adapter, Index 1 (97% over 36bp)
TGCTGCGCAGCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCTC6040990.6394618408956605TruSeq Adapter, Index 1 (97% over 36bp)
GTTGAGCTCAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCTC4546420.4812559038642423TruSeq Adapter, Index 1 (97% over 36bp)
CTATTGCAATAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCTC4017930.4253132209107946TruSeq Adapter, Index 1 (97% over 36bp)
TATCTCGAGATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCTC3546670.375428551818396TruSeq Adapter, Index 1 (97% over 36bp)
ATCGACGTCGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCTC2086500.2208639860401682TruSeq Adapter, Index 1 (97% over 36bp)
GCATTCGAATGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCTC1706280.1806162483108642TruSeq Adapter, Index 1 (97% over 36bp)
GTGAGGCCTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCTC1248600.13216907403295183TruSeq Adapter, Index 1 (97% over 36bp)
CATGACGTCATGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCTC1241290.13139528264164885TruSeq Adapter, Index 1 (97% over 36bp)
TCTCTGCAGAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCTC1234820.13071040845536566TruSeq Adapter, Index 1 (97% over 36bp)
AGGATGCATCCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCTC1139350.12060454468960728TruSeq Adapter, Index 1 (97% over 36bp)
CTCAGCGCTGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCTC982250.1039749102746011TruSeq Adapter, Index 1 (97% over 36bp)
AGAAGGCCTTCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCTC958420.10145241385124273TruSeq Adapter, Index 1 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)