FastQCFastQC Report
Fri 16 Dec 2022
EGAF00007543422

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007543422
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71591746
Sequences flagged as poor quality0
Sequence length51
%GC35

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATAGCTATGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT2395310.33457907284451477Illumina Single End PCR Primer 1 (100% over 39bp)
GCATTCGAATGCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT2121630.29635120227407225Illumina Single End PCR Primer 1 (100% over 39bp)
TGCTGCGCAGCAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT2043100.2853820606638089Illumina Single End PCR Primer 1 (100% over 39bp)
CTATTGCAATAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT1932520.2699361459909079Illumina Single End PCR Primer 1 (100% over 39bp)
GTTGAGCTCAACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT1842180.25731737287144807Illumina Single End PCR Primer 1 (100% over 39bp)
ACAACCGGTTGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT1681360.23485388944138896Illumina Single End PCR Primer 1 (100% over 39bp)
CAAGAGCTCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT1572330.21962448017401334Illumina Single End PCR Primer 1 (100% over 39bp)
TGTAGGACCTACAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC938760.13112684805871336Illumina Single End PCR Primer 1 (100% over 38bp)
TATCTCGAGATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT872170.12182549647552945Illumina Single End PCR Primer 1 (100% over 39bp)
GTGAGGCCTCACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT761160.10631951901270853Illumina Single End PCR Primer 1 (100% over 39bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)