FastQCFastQC Report
Sun 12 May 2024
EGAF00007543424

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007543424
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences139852874
Total Bases7.1 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC35

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATAGCTATGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC40960662.9288393458399717Illumina Single End PCR Primer 1 (100% over 38bp)
TGCTGCGCAGCAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC23572361.685511303829194Illumina Single End PCR Primer 1 (100% over 38bp)
CTATTGCAATAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC12628530.9029868059772586Illumina Single End PCR Primer 1 (100% over 38bp)
GTTGAGCTCAACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC9931890.7101670288162973Illumina Single End PCR Primer 1 (100% over 38bp)
CAAGAGCTCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC4520670.32324469785297366Illumina Single End PCR Primer 1 (100% over 38bp)
GCATTCGAATGCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC4091840.29258175988574964Illumina Single End PCR Primer 1 (100% over 38bp)
TATCTCGAGATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC4016740.2872118309131066Illumina Single End PCR Primer 1 (100% over 38bp)
ACAACCGGTTGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC3771150.2696512336242729Illumina Single End PCR Primer 1 (100% over 38bp)
AGAAGGCCTTCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC3368060.24082880127297207Illumina Single End PCR Primer 1 (100% over 38bp)
CCATAGCTATGAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC3174720.22700427307629015Illumina Single End PCR Primer 1 (100% over 38bp)
CCATAGCTATAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC2840530.20310844666660194Illumina Single End PCR Primer 1 (100% over 38bp)
CCATAGCTATGGAAATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC2768970.1979916408439343Illumina Single End PCR Primer 1 (97% over 38bp)
TGTAGGCCTACAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC2696880.19283693805248506Illumina Single End PCR Primer 1 (100% over 38bp)
CTCAGCGCTGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC1984880.14192629319866534Illumina Single End PCR Primer 1 (100% over 38bp)
AGGATGCATCCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC1905520.13625175840147552Illumina Single End PCR Primer 1 (100% over 38bp)
ATCGACGTCGATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC1426490.10199933395719847Illumina Single End PCR Primer 1 (100% over 38bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)