FastQCFastQC Report
Fri 16 Dec 2022
EGAF00007543424

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007543424
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences139852874
Sequences flagged as poor quality0
Sequence length51
%GC35

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATAGCTATGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT40953592.928333814577168Illumina Single End PCR Primer 1 (100% over 39bp)
TGCTGCGCAGCAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT23566541.685095152209743Illumina Single End PCR Primer 1 (100% over 39bp)
CTATTGCAATAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT12626870.9028681098108859Illumina Single End PCR Primer 1 (100% over 39bp)
GTTGAGCTCAACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT9929500.7099961349382066Illumina Single End PCR Primer 1 (100% over 39bp)
CAAGAGCTCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT4519680.3231739091754382Illumina Single End PCR Primer 1 (100% over 39bp)
GCATTCGAATGCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT4091150.29253242232261883Illumina Single End PCR Primer 1 (100% over 39bp)
TATCTCGAGATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT4015970.2871567730528012Illumina Single End PCR Primer 1 (100% over 39bp)
ACAACCGGTTGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT3770410.26959832087540797Illumina Single End PCR Primer 1 (100% over 39bp)
AGAAGGCCTTCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT3367460.24078589904416264Illumina Single End PCR Primer 1 (100% over 39bp)
CCATAGCTATGAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT3174150.22696351595892122Illumina Single End PCR Primer 1 (100% over 39bp)
CCATAGCTATAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT2839940.20306625947493936Illumina Single End PCR Primer 1 (100% over 39bp)
CCATAGCTATGGAAATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT2768510.19795874913518044Illumina Single End PCR Primer 1 (97% over 39bp)
TGTAGGCCTACAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT2696380.19280118619514391Illumina Single End PCR Primer 1 (100% over 39bp)
CTCAGCGCTGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT1984580.14190484208426063Illumina Single End PCR Primer 1 (100% over 39bp)
AGGATGCATCCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT1905290.13623531254709859Illumina Single End PCR Primer 1 (100% over 39bp)
ATCGACGTCGATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT1425970.10196215202556366Illumina Single End PCR Primer 1 (100% over 39bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)