FastQCFastQC Report
Fri 16 Dec 2022
EGAF00007543425

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007543425
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57115318
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGAGCTCAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAGG20530603.594587357458116TruSeq Adapter, Index 2 (97% over 38bp)
CTATTGCAATAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAGG19855583.476401899749556TruSeq Adapter, Index 2 (97% over 38bp)
TGCTGCGCAGCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAGG17459763.0569312421581896TruSeq Adapter, Index 2 (97% over 38bp)
CCATAGCTATGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAGG9935151.7394895709063547TruSeq Adapter, Index 2 (97% over 38bp)
ACAACCGGTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAGG5866121.02706597904261TruSeq Adapter, Index 2 (97% over 38bp)
TGTAGGCCTACAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAGG3725490.6522751042023438TruSeq Adapter, Index 2 (97% over 38bp)
CAAGAGCTCTTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAGG3484340.6100535061364798TruSeq Adapter, Index 2 (97% over 38bp)
TATCTCGAGATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAGG2363050.4137331424820221TruSeq Adapter, Index 2 (97% over 38bp)
AGGATGCATCCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAGG1668820.292184313847294TruSeq Adapter, Index 2 (97% over 38bp)
ATCGACGTCGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAGG1651410.28913609480384933TruSeq Adapter, Index 2 (97% over 38bp)
GTTGAGCTCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAGG1521480.2663873814026563TruSeq Adapter, Index 2 (97% over 38bp)
GCATTCGAATGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAGG1521150.26632960355749047TruSeq Adapter, Index 2 (97% over 38bp)
GTTGAGCTTAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAGG1338460.23434343830493948TruSeq Adapter, Index 2 (97% over 38bp)
CTCAGCGCTGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAGG1334160.23359057547399104TruSeq Adapter, Index 2 (97% over 38bp)
TGTTGCGCAGCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAGG1296500.22699689774991713TruSeq Adapter, Index 2 (97% over 38bp)
TCATAGCTATGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAGG1131070.19803268888391726TruSeq Adapter, Index 2 (97% over 38bp)
AGAAGGCCTTCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAGG1116260.19543968922662744TruSeq Adapter, Index 2 (97% over 38bp)
TGCTGCGCAGTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAGG1104280.19334217836272924TruSeq Adapter, Index 2 (97% over 38bp)
TTATTGCAATAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAGG1076380.18845732418052893TruSeq Adapter, Index 2 (97% over 38bp)
CATGACGTCATGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAGG1047870.18346566852696153TruSeq Adapter, Index 2 (97% over 38bp)
CTATTGGATCGGAAGAGCACACGTCTGAACCAATAGAGATCGGAAGAGCAC977630.17116774172560853Illumina Multiplexing PCR Primer 2.01 (96% over 25bp)
CTATAGCTATGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAGG974490.1706179767746369TruSeq Adapter, Index 2 (97% over 38bp)
GTGAGGCCTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAGG917480.160636416311295TruSeq Adapter, Index 2 (97% over 38bp)
CTATTGGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAGGCTGATCT757050.13254762934174683TruSeq Adapter, Index 2 (97% over 38bp)
ACTCACGTGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAGG674920.11816794927063175TruSeq Adapter, Index 2 (97% over 38bp)
TGTAGGTTCGGAAGAGCACACGTCTGAACTCCAGTCACCGAGGCTGATCTC672720.11778276363619301TruSeq Adapter, Index 2 (97% over 36bp)
GGTAACGTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAGG624600.10935770330474216TruSeq Adapter, Index 2 (97% over 38bp)
ATCAAGCTTGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAGG610810.10694328971432847TruSeq Adapter, Index 2 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)