FastQCFastQC Report
Fri 16 Dec 2022
EGAF00007543427

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007543427
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79117137
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTGCGCAGCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGAG17284982.1847327463328203TruSeq Adapter, Index 7 (97% over 37bp)
CTATTGCAATAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGAG12422051.5700833562771614TruSeq Adapter, Index 7 (97% over 37bp)
ACAACCGGTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGAG11337481.4329992754919836TruSeq Adapter, Index 7 (97% over 37bp)
CCATAGCTATGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGAG9623951.2164178792263427TruSeq Adapter, Index 7 (97% over 37bp)
GTTGAGCTCAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGAG8877561.122078014526739TruSeq Adapter, Index 7 (97% over 37bp)
CAAGAGCTCTTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGAG7370990.9316552999130897TruSeq Adapter, Index 7 (97% over 37bp)
TGTAGGCCTACAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGAG6462680.8168495783663153TruSeq Adapter, Index 7 (97% over 37bp)
GCATTCGAATGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGAG5768420.7290986780777975TruSeq Adapter, Index 7 (97% over 37bp)
TATCTCGAGATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGAG3303570.4175542904187749TruSeq Adapter, Index 7 (97% over 37bp)
GTGAGGCCTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGAG2778970.3512475432471729TruSeq Adapter, Index 7 (97% over 37bp)
AGGATGCATCCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGAG2310870.29208210605497514TruSeq Adapter, Index 7 (97% over 37bp)
CTCAGCGCTGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGAG2263670.28611626833766746TruSeq Adapter, Index 7 (97% over 37bp)
AGAAGGCCTTCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGAG1522130.19238941874249066TruSeq Adapter, Index 7 (97% over 37bp)
ATCGACGTCGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGAG1359270.17180475072044127TruSeq Adapter, Index 7 (97% over 37bp)
ATCAAGCTTGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGAG1206510.15249667085400223TruSeq Adapter, Index 7 (97% over 37bp)
TCATAGCTATGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGAG1182700.14948720907330101TruSeq Adapter, Index 7 (97% over 37bp)
CATGACGTCATGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGAG1157380.14628689104359274TruSeq Adapter, Index 7 (97% over 37bp)
TGTTGCGCAGCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGAG1147780.14507350032142846TruSeq Adapter, Index 7 (97% over 37bp)
CTATAGCTATGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGAG1043470.13188925175591223TruSeq Adapter, Index 7 (97% over 37bp)
TGCTGCGCAGTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGAG992250.12541530667369827TruSeq Adapter, Index 7 (97% over 37bp)
GTATTCGAATGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGAG917490.11596602642484397TruSeq Adapter, Index 7 (97% over 37bp)
GCATTCGAATGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGAG849740.10740277419290335TruSeq Adapter, Index 7 (97% over 37bp)
TGTAGGTTCGGAAGAGCACACGTCTGAACTCCAGTCACAAGAGGCAATCTC832070.10516937689491976TruSeq Adapter, Index 1 (97% over 34bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)