FastQCFastQC Report
Fri 16 Dec 2022
EGAF00007543429

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007543429
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86708470
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTGCGCAGCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGA5788510.6675829939105142TruSeq Adapter, Index 3 (97% over 37bp)
ACAACCGGTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGA5694830.6567789744185314TruSeq Adapter, Index 3 (97% over 37bp)
CCATAGCTATGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGA4965230.5726349455825942TruSeq Adapter, Index 3 (97% over 37bp)
CTATTGCAATAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGA4192060.48346603278780037TruSeq Adapter, Index 3 (97% over 37bp)
GTTGAGCTCAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGA3702450.4269998075159208TruSeq Adapter, Index 3 (97% over 37bp)
CAAGAGCTCTTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGA2942520.33935785050756867TruSeq Adapter, Index 3 (97% over 37bp)
TATCTCGAGATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGA1927910.22234390711772448TruSeq Adapter, Index 3 (97% over 37bp)
AGGATGCATCCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGA1169190.1348414981835108TruSeq Adapter, Index 3 (97% over 37bp)
GTGAGGCCTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGA1120850.12926649495718237TruSeq Adapter, Index 3 (97% over 37bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)