FastQCFastQC Report
Mon 13 May 2024
EGAF00007543433

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007543433
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences131417134
Total Bases6.7 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC35

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCAAGTTCGGAAGAGCACACGTCTGAACTTGATAGATCGGAAGAGCACA2427340.18470498679418773Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
AGAAGGTCCTTCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAG1690740.12865445688383373TruSeq Adapter, Index 10 (100% over 36bp)
GTTGAGTTCGGAAGAGCACACGTCTGAACTCAACAGATCGGAAGAGCACA1596070.12145067780887689Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)