FastQCFastQC Report
Fri 16 Dec 2022
EGAF00007543436

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007543436
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences123366423
Sequences flagged as poor quality0
Sequence length51
%GC32

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTAGGTTCAGACGTGTGCTCTTCCGAACCTACAAGATCGGAAGAGCGTCG1743130.14129695565542982Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
ATCAAGTTCAGACGTGTGCTCTTCCGAACTTGATAGATCGGAAGAGCGTCG1531220.12411967233580243Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
ACAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG1287170.1043371420439093Illumina Single End PCR Primer 1 (100% over 48bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)