FastQCFastQC Report
Fri 16 Dec 2022
EGAF00007543439

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007543439
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences99663844
Sequences flagged as poor quality0
Sequence length51
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTGCGCAGCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTAC13515791.3561377383758146TruSeq Adapter, Index 6 (97% over 37bp)
CAAGAGCTCTTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTAC9231560.9262697112104165TruSeq Adapter, Index 6 (97% over 37bp)
CTATTGCAATAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTAC8149600.8177087771168048TruSeq Adapter, Index 6 (97% over 37bp)
ACAACCGGTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTAC5972710.5992855342806164TruSeq Adapter, Index 6 (97% over 37bp)
GTTGAGCTCAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTAC5971400.5991540924309522TruSeq Adapter, Index 6 (97% over 37bp)
CCATAGCTATGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTAC5407950.5426190464818916TruSeq Adapter, Index 6 (97% over 37bp)
CATGACGTCATGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTAC3057180.3067491556918074TruSeq Adapter, Index 6 (97% over 37bp)
TGTAGGCCTACAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTAC2907510.29173167352445284TruSeq Adapter, Index 6 (97% over 37bp)
GCATTCGAATGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTAC2888200.28979416045802925TruSeq Adapter, Index 6 (97% over 37bp)
GTGAGGCCTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTAC2524810.2533325927103514TruSeq Adapter, Index 6 (97% over 37bp)
TATCTCGAGATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTAC1613880.16193234529464867TruSeq Adapter, Index 6 (97% over 37bp)
AGGATGCATCCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTAC1424260.1429063883989865TruSeq Adapter, Index 6 (97% over 37bp)
ACTCACGTGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTAC1406520.14112640487758027TruSeq Adapter, Index 6 (97% over 37bp)
TCTCTGCAGAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTAC1406040.1410782429784667TruSeq Adapter, Index 6 (97% over 37bp)
TGACAGCTGTCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTAC1380420.13850760161327913TruSeq Adapter, Index 6 (97% over 37bp)
CTCAGCGCTGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTAC1200670.12047197376814003TruSeq Adapter, Index 6 (97% over 37bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)