FastQCFastQC Report
Fri 16 Dec 2022
EGAF00007543443

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007543443
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90148325
Sequences flagged as poor quality0
Sequence length51
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGAGCTCAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGAG3699920.41042581767326236TruSeq Adapter, Index 7 (97% over 37bp)
ACAACCGGTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGAG3226110.357866882163368TruSeq Adapter, Index 7 (97% over 37bp)
CCATAGCTATGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGAG3192130.35409753869525584TruSeq Adapter, Index 7 (97% over 37bp)
CTATTGCAATAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGAG2742600.3042319421908283TruSeq Adapter, Index 7 (97% over 37bp)
TGCTGCGCAGCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGAG2676080.29685299199957405TruSeq Adapter, Index 7 (97% over 37bp)
CAAGAGCTCTTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGAG2196450.24364845381209246TruSeq Adapter, Index 7 (97% over 37bp)
TGTAGGCCTACAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGAG2009850.22294923394305996TruSeq Adapter, Index 7 (97% over 37bp)
TATCTCGAGATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGAG1251170.13879015500287997TruSeq Adapter, Index 7 (97% over 37bp)
GTGAGGCCTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGAG1239220.137464561876219TruSeq Adapter, Index 7 (97% over 37bp)
GCATTCGAATGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGAG913750.10136072966413963TruSeq Adapter, Index 7 (97% over 37bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)