FastQCFastQC Report
Fri 16 Dec 2022
EGAF00007543444

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007543444
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90148325
Sequences flagged as poor quality0
Sequence length51
%GC32

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGAGCTCAACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT3798130.4213200855368084Illumina Single End PCR Primer 1 (100% over 39bp)
ACAACCGGTTGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT3214280.35655460043212117Illumina Single End PCR Primer 1 (100% over 39bp)
CCATAGCTATGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT3094780.34329866916551144Illumina Single End PCR Primer 1 (100% over 39bp)
TGCTGCGCAGCAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT2804790.31113057286422124Illumina Single End PCR Primer 1 (100% over 39bp)
CTATTGCAATAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT2708570.30045705230796027Illumina Single End PCR Primer 1 (100% over 39bp)
CAAGAGCTCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT2171450.24087524643414063Illumina Single End PCR Primer 1 (100% over 39bp)
TGTAGGCCTACAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT2068260.2294285556609066Illumina Single End PCR Primer 1 (100% over 39bp)
GTGAGGCCTCACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT1290140.1431130306636313Illumina Single End PCR Primer 1 (100% over 39bp)
TATCTCGAGATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT1251370.1388123406619036Illumina Single End PCR Primer 1 (100% over 39bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)