FastQCFastQC Report
Fri 16 Dec 2022
EGAF00007543445

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007543445
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85348896
Sequences flagged as poor quality0
Sequence length51
%GC35

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTGCGCAGCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGA13975031.6374002072622005TruSeq Adapter, Index 3 (97% over 37bp)
CCATAGCTATGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGA9859811.1552357982462949TruSeq Adapter, Index 3 (97% over 37bp)
ACAACCGGTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGA4771230.5590265631555444TruSeq Adapter, Index 3 (97% over 37bp)
GTTGAGCTCAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGA3780420.44293718808032384TruSeq Adapter, Index 3 (97% over 37bp)
TGTAGGCCTACAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGA3190500.37381854359311223TruSeq Adapter, Index 3 (97% over 37bp)
CAAGAGCTCTTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGA2790580.3269614641529751TruSeq Adapter, Index 3 (97% over 37bp)
CTATTGCAATAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGA2514790.29464821665648727TruSeq Adapter, Index 3 (97% over 37bp)
TATCTCGAGATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGA2190870.25669576323517995TruSeq Adapter, Index 3 (97% over 37bp)
GTGAGGCCTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGA2190510.2566535834277224TruSeq Adapter, Index 3 (97% over 37bp)
AGGATGCATCCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGA1876040.21980834995217746TruSeq Adapter, Index 3 (97% over 37bp)
AGAAGGCCTTCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGA1590010.18629532126578416TruSeq Adapter, Index 3 (97% over 37bp)
TCATAGCTATGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGA986320.11556329914331875TruSeq Adapter, Index 3 (97% over 37bp)
CTATAGCTATGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGA906730.10623804671123104TruSeq Adapter, Index 3 (97% over 37bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)