FastQCFastQC Report
Fri 16 Dec 2022
EGAF00007543446

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007543446
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85348896
Sequences flagged as poor quality0
Sequence length51
%GC33

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTGCGCAGCAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT14443991.6923464364436536Illumina Single End PCR Primer 1 (100% over 39bp)
CCATAGCTATGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT9429451.1048121817533527Illumina Single End PCR Primer 1 (100% over 39bp)
ACAACCGGTTGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT4749700.5565039763373154Illumina Single End PCR Primer 1 (100% over 39bp)
GTTGAGCTCAACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT3860000.4522612688510933Illumina Single End PCR Primer 1 (100% over 39bp)
TGTAGGCCTACAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT3259470.38189949170520027Illumina Single End PCR Primer 1 (100% over 39bp)
CAAGAGCTCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT2742730.3213550647450671Illumina Single End PCR Primer 1 (100% over 39bp)
CTATTGCAATAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT2484600.291110971136639Illumina Single End PCR Primer 1 (100% over 39bp)
GTGAGGCCTCACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT2274830.2665330316633504Illumina Single End PCR Primer 1 (100% over 39bp)
TATCTCGAGATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT2188800.2564532293422987Illumina Single End PCR Primer 1 (100% over 39bp)
AGGATGCATCCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT1864110.21841055799948486Illumina Single End PCR Primer 1 (100% over 39bp)
AGAAGGCCTTCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT1589730.18626251474887268Illumina Single End PCR Primer 1 (100% over 39bp)
CCATAGCTATGAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT982150.11507471637360137Illumina Single End PCR Primer 1 (100% over 39bp)
CCATAGCTATAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT897670.10517652155688106Illumina Single End PCR Primer 1 (100% over 39bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)