FastQCFastQC Report
Fri 10 May 2024
EGAF00007543612

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007543612
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57717
Total Bases2.9 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC33

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38966.750177590657865No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13252.295684113865932No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA12182.110296792972608No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA8061.3964689779441066No Hit
GAGTGAATGGGGCGGTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6581.1400453939047421No Hit
GAGTGAATGGGGCGGTTCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6121.0603461718384533No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA5380.932134379818771No Hit
GTGAATGGGGAGCGGTTCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4840.8385744234800839No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA4660.8073877713671882No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAC4070.7051648561082523No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC3870.6705130204272571No Hit
GAGTGAATGGGGAGCGGTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3810.6601174697229586No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC3380.5856160230088189No Hit
GTGAATGGGGAGCGGTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3290.5700226969523711No Hit
GAGTGAATGGGGAGCGGTTCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAA3230.5596271462480724No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAC3070.5319056777032763No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC3000.519777535214928No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCC2280.3950309267633453No Hit
GATCGGAAGAGCGGTTCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2210.38290278427499697Illumina Paired End Adapter 2 (95% over 21bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAC2200.3811701924909472No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTAACC2030.3517161321621013No Hit
GAGTGAATGGGGCGGTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT2010.3482509485940018No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAACC2010.3482509485940018No Hit
GATCGGAAGAGCGGTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1770.3066687457768075No Hit
AGTGAATGGGGAGCGGTTCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1720.29800578685655876No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA1690.29280801150440944No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC1670.28934282793630994No Hit
AGTGAATGGGGAGCGGTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1630.2824124608001109No Hit
GAGTGAATGGGAGCGGTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1580.2737495018798621No Hit
GAGTGAATGGGCGGTTCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1500.259888767607464No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCC1500.259888767607464No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCCC1490.25815617582341427No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAC1480.25642358403936444No Hit
GTGAATGGGGCGGTTCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1390.24083025798291666No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG1380.23909766619886688No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC1340.23216729906266786No Hit
GAGTGAATGGGAGCGGTTCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1260.21830656479026977No Hit
AGTGAATGGGGCGGTTCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1230.21310878943812048No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC1220.21137619765407073No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1190.20617842230192143No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTACC1170.20271323873382194No Hit
GAGTGAATGGGCGGTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1150.1992480551657224No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA1130.1957828715976229No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCCC1100.1905850962454736No Hit
AGTGAATGGGGCGGTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1030.17845695375712528No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTAACCC1030.17845695375712528No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGA990.17152658662092624No Hit
GTGAATGGGGAGCGGTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT990.17152658662092624No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAC960.16632881126877697No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTACC940.16286362770067744No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTAACCC930.1611310359166277No Hit
GAGTGAATGGGGCGGTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATA920.15939844413257792No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAC870.15073548521232913No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAACC830.14380511807613008No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAACCC720.12474660845158272No Hit
CTCCATGGGGAACTTTACTTTCATTACGCCTATAAGTTTTAACACTCATT710.12301401666753296No Hit
GAGTGAATGGGGCGGTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC680.11781624131538368No Hit
GTGAATGGGGCGGTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA640.11088587417918463No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTAAACC640.11088587417918463No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTCCCCCC630.10915328239513489No Hit
GAGTGAATGGGGCGGTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT620.10742069061108511No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAC610.10568809882703536No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG600.10395550704298562No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTACCC600.10395550704298562No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCCCC580.1004903234748861No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)