FastQCFastQC Report
Thu 15 Dec 2022
EGAF00007543618

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007543618
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63267
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15812.4989330930817015No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA5220.8250746834842809No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4800.7586893641234767No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA4070.6433053566630313No Hit
CTCATCATCGAGGCTATATATTAATAGACATGGTATTAAGCCCACACGAAA2980.47101964689332515No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC2710.4283433701613795No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAC2630.4156985474259882No Hit
GTGAATGGGGAGCGGTTCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2460.3888282991132818No Hit
GAGTGAATGGGGCGGTTCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2430.3840864905875101No Hit
GAGTGAATGGGGCGGTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2190.34615202238133624No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCC2190.34615202238133624No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC2180.34457141953941234No Hit
CCTAAAGTAGACTTCATTTTTCCTAACAGGATTAGACAGTCAAGGAGTGGC1950.3082175541751624No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA1950.3082175541751624No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA1910.30189514280746677No Hit
CCCCGAGGACGGAGATGAGGAGTAGTCAGCGTGATGCCAAAACGCGTCTTC1890.29873393712361895No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC1870.29557273143977114No Hit
GAGTGAATGGGGAGCGGTTCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1820.28766971723015156No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAC1630.2576382632335973No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAACC1630.2576382632335973No Hit
GAGTGAATGGGGAGCGGTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1620.2560576603916734No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAC1620.2560576603916734No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAACC1440.227606809237043No Hit
GTGAATGGGGAGCGGTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1310.20705897229203218No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCCC1290.20389776660818437No Hit
CCCAGAGGCACGGATCCAAGTACCTGGCCACAGCAAGTACCATGGACCATG1280.20231716376626044No Hit
CACATAGGAAACAGGGTCTTCCTGGAGATTTGGTGATGGAGATGTCAAGCA1240.1959947523985648No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCC1200.18967234103086916No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTACC1170.18493053250509744No Hit
CCCCTAATGCCTGCGGAGTTGTGCACGTAGTAGGGTCAGGCCACGGCAGCC1140.18018872397932573No Hit
ACTTTAGGAGGTGAGAGAAGGACAGGAAAAAAAAACAAAGGAAGCTTGGAT1100.17386631261163008No Hit
GATCGGAAGAGCGGTTCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1080.17070510692778226Illumina Paired End Adapter 2 (95% over 21bp)
CCTACTGACCCACCTTTTGATTTTAGCGCACCTGTGATTGATAGGCCTTCC1080.17070510692778226No Hit
CCCTGAGAAGATAACCGGCTCATTCACTTCCTCCCAGAAGACGCGTGGTAG1060.16754390124393445No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAC1040.1643826955600866No Hit
ACTACATGTGGGAGCTTTTGGTATGTGACATGCGGGCTGGGCAGCTGTTAG990.15647968135046708No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCCC980.15489907850854318No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC910.14383485861507578No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA900.1422542557731519No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTAACCC890.14067365293122797No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAACCC860.13593184440545625No Hit
ACCTCAGCTCAGCGACGCAGAGTGCTTCTGCTGAAAAATTTGGAGGTTTGT850.13435124156353234No Hit
AGTGAATGGGGAGCGGTTCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA830.1311900358796845No Hit
ACACGAAACATTCAGAATTAGAATTGGATTAAGAAGACGCGTTTTGGCATC790.12486762451198888No Hit
CCACCCTTGGACTTTCTTTTCGCCGTGGCTGCGGCACCCTTGCGCTTTTGC790.12486762451198888No Hit
GTTTCCTATGTGAGGCCATTGCCCAGCCCACGTTTGCCTCCTTTTCCTCCC790.12486762451198888No Hit
GCTCTGGGGCCACCATGCAGGGCAACGGTGACGTCCAGAGGCCACCTGCTT770.12170641882814105No Hit
AGTGAATGGGGAGCGGTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA750.11854521314429323No Hit
GGCCACAGCAAGTACCATGGACCATGCCAGGCATGGCTTCCTCCCAAGGCA730.1153840074604454No Hit
GAGTGAATGGGCGGTTCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA700.1106421989346737No Hit
GCGACGGGGGTGGTGTGCGAGGCGTCACAATGTTCTTGAAGAAGTGGACTA700.1106421989346737No Hit
GTTATCTTCTCAGGGAGGGTTAGTGCTGGCCACTGTGCACTGCCGCTGCCA690.10906159609274978No Hit
GAGTGAATGGGAGCGGTTCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA690.10906159609274978No Hit
GTGATTGATAGGCCTTCCAAAGAGTCCCACGCTGGCATCACCCTCCCCGAG670.10590039040890195No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTACC660.10431978756697805No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC660.10431978756697805No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC660.10431978756697805No Hit
GCTGTGTGGGTGCGGCCACGTATGGATCGCAGAGCCGACCTCAGCTCAGCG660.10431978756697805No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAC650.10273918472505414No Hit
GTAGTCCACTTCTTCAAGAACATTGTGACGCCTCGCACACCACCCCCGTCG650.10273918472505414No Hit
AGTGAATGGGGCGGTTCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA650.10273918472505414No Hit
CCCCCGTCGCAGGGAAAGGGGAGAGGACTGTCCCTGAGCAGATTTAGCTGG650.10273918472505414No Hit
GATCGGAAGAGCGGTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA640.10115858188313022No Hit
CCCAGAAGACGCGTGGTAGCGAGTAGGCACAGGCGTGCACCTGCTCCCGAA640.10115858188313022No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)