FastQCFastQC Report
Thu 15 Dec 2022
EGAF00007543651

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007543651
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52955
Sequences flagged as poor quality0
Sequence length51
%GC32

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20893.94485884241337No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6841.291662732508734No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA6331.1953545463129072No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA5611.059390048154093No Hit
GAGTGAATGGGGCGGTTCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4350.8214521763761685No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAC4150.7836842602209423No Hit
GAGTGAATGGGGCGGTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3860.7289207817958644No Hit
GTGAATGGGGAGCGGTTCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3490.6590501369086961No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCC3340.6307241997922765No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC3270.6175054291379473No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC2800.5287508261731658No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAACC2660.5023132848645075No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC2630.49664809744122373No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA2570.48531772259465583No Hit
GAGTGAATGGGGAGCGGTTCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2540.4796525351713719No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAC2500.4720989519403267No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAC2380.44943820224719105No Hit
GAGTGAATGGGGAGCGGTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2330.43999622320838444No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA2300.4343310357851005No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAACC2190.41355868189972617No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCCC2080.3927863280143518No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTACC2070.3908979322065905No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTAACCC1900.35879520347464827No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAACCC1570.29647814181852516No Hit
GTGAATGGGGAGCGGTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1480.2794825795486734No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCC1440.2719289963176282No Hit
GGTATCAACGCAGAGTGAATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA1420.26815220470210555No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAC1340.2530450382400151No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG1270.23982626758568595No Hit
AGTGAATGGGGAGCGGTTCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1260.23793787177792464No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA1260.23793787177792464No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTACC1230.23227268435464074No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAC1180.2228307053158342No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTACCC1180.2228307053158342No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC1140.215277122084789No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCCC1120.21150033046926636No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC1110.20961193466150504No Hit
GAGTGAATGGGGCGGTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT1090.20583514304598244No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC1090.20583514304598244No Hit
GGTATCAACGCAGAGTGAATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA1000.18883958077613067No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTACCC990.18695118496836938No Hit
GTATCAACGCAGAGTGAATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA970.18317439335284674No Hit
AGTGAATGGGGCGGTTCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA950.17939760173732414No Hit
AGTGAATGGGGAGCGGTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA920.1737324143140402No Hit
GAGTGAATGGGCGGTTCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA910.17184401850627892No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT900.1699556226985176No Hit
GAGTGAATGGGAGCGGTTCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA890.16806722689075632No Hit
GAGTGAATGGGGCGGTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC880.166178831082995No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTAACCCC870.16429043527523368No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTAACCCC870.16429043527523368No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGA850.16051364365971107No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAACC830.15673685204418847No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTACCCC820.15484845623642715No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAC810.15296006042866586No Hit
GAGTGAATGGGAGCGGTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA800.15107166462090454No Hit
GATCGGAAGAGCGGTTCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA790.14918326881314323Illumina Paired End Adapter 2 (95% over 21bp)
GTATCAACGCAGAGTGAATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA770.14540647719762062No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCCCC760.1435180813898593No Hit
GAGTGAATGGGCGGTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA720.1359644981588141No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTACCCC720.1359644981588141No Hit
AGTGAATGGGGCGGTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA710.13407610235105277No Hit
GTGAATGGGGCGGTTCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA700.13218770654329146No Hit
GTATCAACGCAGAGTGAATGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAA660.12463412331224626No Hit
GAGTGAATGGGGCGGTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATA660.12463412331224626No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAACCCC610.11519214427343971No Hit
GGTATCAACGCAGAGTGAATGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAA600.11330374846567841No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTAACCCCC560.10575016523463318No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCCCC560.10575016523463318No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGAA550.10386176942687188No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA550.10386176942687188No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)