FastQCFastQC Report
Thu 15 Dec 2022
EGAF00007543653

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007543653
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences254315
Sequences flagged as poor quality0
Sequence length51
%GC62

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCCCAGTTCCTGCACCCTTCTCTCCCTGTCTCTTATACACATCTCCGAG90743.56801604309616No Hit
CCTGAGCCACTACCATGCTGCTATACGAGAGACTGTCTCTTATACACATCT81673.2113717240430177No Hit
TACCCCAGTTCCTGCACTCTTCTCTCCCTGTCTCTTATACACATCTCCGAG28681.1277352889133554No Hit
CCTGAGCCACTACCATGCTGTCTCTTATACACATCTCCGAGCCCACGAGAC26551.0439808898413385No Hit
CATCATGGGCCAGAATGTTGCTGTCTCTTATACACATCTCCGAGCCCACGA14340.5638676444566777No Hit
TACCCCAGTTCCTGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCG13310.5233666909148104No Hit
TACCCCAGTTCCTGCTTCTGCTCCCTGTCTCTTATACACATCTCCGAGCCC11370.4470833415252738No Hit
CCTGAGCCACTACCATGCTGCTATACGAGCTGTCTCTTATACACATCTCCG9960.3916402886184456No Hit
TACCCCAGTTCCTGCACCCTTCTGCTCCCTGTCTCTTATACACATCTCCGA9900.38928100977134655No Hit
AGTTCCTGCACCCTTCTCTCCCTGTCTCTTATACACATCTCCGAGCCCACG9800.3853488783595148No Hit
CGGAGATGTGTATAAGAGACAGTGGGTCTGGCTCTTATACACATCTCCGAG6640.2610935257456304No Hit
GCTATACGAGAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAG6230.24497178695712013No Hit
GCGTCAGATGTGTATAAGAGACAGCCTGAGCCACTACCATGCTGCTATACG5280.20761653854471815No Hit
TACCCCAGTTCCTGCACTCTCGCTGTCTCTTATACACATCTCCGAGCCCAC5240.20604368597998546No Hit
TCTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCC5050.19857263629750507No Hit
GCGTCAGATGTGTATAAGAGACAGTACCCCAGTTCCTGCACCCTTCTCTCC4640.18245089750899474No Hit
TACCCCAGTTCCTGCACTCTTCTGCTCCCTGTCTCTTATACACATCTCCGA4080.16043096160273676No Hit
TACCCCAGTTCCTGCACCCTTCTCTCGCTGTCTCTTATACACATCTCCGAG3500.13762459941411243No Hit
CTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGAA3420.134478894284647RNA PCR Primer, Index 10 (96% over 28bp)
CCTGAGCCACTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTG3080.12110964748441892No Hit
GAGCCACTACCATGCTGCTATACGAGAGACTGTCTCTTATACACATCTCCG3030.11914358177850305No Hit
CAGTTCCTGCACCCTTCTCTCCCTGTCTCTTATACACATCTCCGAGCCCAC2760.10852682696655722No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)