FastQCFastQC Report
Thu 15 Dec 2022
EGAF00007543698

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007543698
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences88564
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCCCAGTTCCTGCACCCTTCTCTCCCTGTCTCTTATACACATCTCCGAG80239.058985592340003No Hit
CCTGAGCCACTACCATGCTGCTATACGAGAGACTGTCTCTTATACACATCT65347.377715550336481No Hit
CCTGAGCCACTACCATGCTGTCTCTTATACACATCTCCGAGCCCACGAGAC20292.290998599882571No Hit
TACCCCAGTTCCTGCACTCTTCTCTCCCTGTCTCTTATACACATCTCCGAG18182.052752811526128No Hit
CATCATGGGCCAGAATGTTGCTGTCTCTTATACACATCTCCGAGCCCACGA10911.2318775123074839No Hit
TACCCCAGTTCCTGCTTCTGCTCCCTGTCTCTTATACACATCTCCGAGCCC10051.1347725938304503No Hit
TACCCCAGTTCCTGCACCCTTCTGCTCCCTGTCTCTTATACACATCTCCGA7750.8750733932523372No Hit
AGTTCCTGCACCCTTCTCTCCCTGTCTCTTATACACATCTCCGAGCCCACG5940.6707014136669527No Hit
TACCCCAGTTCCTGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCT5580.6300528431416829No Hit
TACCCCAGTTCCTGCACTCTCGCTGTCTCTTATACACATCTCCGAGCCCAC4590.5182692741971907No Hit
CCTGAGCCACTACCATGCTGCTATACGAGCTGTCTCTTATACACATCTCCG4310.48665371934420304No Hit
TCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCC4290.4843954654261325No Hit
GCTATACGAGAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCT3170.35793324601418186No Hit
TACCCCAGTTCCTGCACCCTTCTCTCGCTGTCTCTTATACACATCTCCGAG3060.3455128494647938No Hit
TACCCCAGTTCCTGCACTCTTCTGCTCCCTGTCTCTTATACACATCTCCGA2720.3071225328575945No Hit
CACCAAGTGCCATCTGGTAGCTGCTGTCTCTTATACACATCTCCGAGCCCA2530.2856691206359243No Hit
CCTGAGCCACTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTAC2060.23260015356126643No Hit
CCAAGGTCAGCAGGGGAAGGGGACACCAGCCACACTTCACCACAGGCATAG1780.20098459870827876No Hit
GCTGAGCCACTACCATGCTGCTATACGAGAGACTGTCTCTTATACACATCT1550.17501467865046744No Hit
CAGTTCCTGCACCCTTCTCTCCCTGTCTCTTATACACATCTCCGAGCCCAC1490.1682399168962558No Hit
GTTCCTGCACCCTTCTCTCCCTGTCTCTTATACACATCTCCGAGCCCACGA1450.16372340906011473No Hit
TACCCCAGTTCCTGCACTCTCCCTGTCTCTTATACACATCTCCGAGCCCAC1310.1479156316336209No Hit
TACCCCAGTTCCTGCACCCTTCTCTCACTGTCTCTTATACACATCTCCGAG1290.14565737771555035No Hit
CCAAAATCAAGTGGGGCGATGCTGGCGCTGAGTACGTCGTGGAGTCCACTG1250.14114086987940924No Hit
CATCATGGGCCAGGATGAGAGCTGTCTCTTATACACATCTCCGAGCCCACG1190.1343661081251976No Hit
CCTGCAAGAAGAGCTGCTGCTCCTGCTGCCCTGCGGAGTGTGAGAAGTGTG1180.13323698116616234No Hit
GCCCCAGAGAACTGCTTCCCACACTCTCGCTGTCTCTTATACACATCTCCG1110.1253330924529154No Hit
TACCCCAGTTCCTGCACTCTTCTCTCGCTGTCTCTTATACACATCTCCGAG1070.1208165846167743No Hit
NACCCCAGTTCCTGCACCCTTCTCTCCCTGTCTCTTATACACATCTCCGAG1040.11742920373966849No Hit
GCCCCAGTTCCTGCACCCTTCTCTCCCTGTCTCTTATACACATCTCCGAGC1030.11630007678063321No Hit
NCTGAGCCACTACCATGCTGCTATACGAGAGACTGTCTCTTATACACATCT1030.11630007678063321No Hit
TACCCCAGTTCCCACACTCTCGCTGTCTCTTATACACATCTCCGAGCCCAC1010.11404182286256268No Hit
CACCAAGTGCCAACTGGTAGCTGCTGTCTCTTATACACATCTCCGAGCCCA1010.11404182286256268No Hit
TCTGAGCCACTACCATGCTGCTATACGAGAGACTGTCTCTTATACACATCT920.10387968023124519No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)