FastQCFastQC Report
Fri 16 Dec 2022
EGAF00007543752

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007543752
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51826233
Sequences flagged as poor quality0
Sequence length51
%GC36

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAAGGTCCTTCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTA3821160.7373022847329074TruSeq Adapter, Index 22 (100% over 37bp)
TGTAGGTCCTACAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTA2943240.5679054466489972TruSeq Adapter, Index 22 (100% over 37bp)
GAAGTCTGACTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTA1413260.272692016801607TruSeq Adapter, Index 22 (100% over 37bp)
CCATAGTCTATGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTA1280660.24710651843054074TruSeq Adapter, Index 22 (100% over 37bp)
CGGTAGTCTACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTA1106860.21357137803166207TruSeq Adapter, Index 22 (100% over 37bp)
ATCAAGTCTTGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTA646930.1248267455595316TruSeq Adapter, Index 22 (100% over 37bp)
CAAGAGTCTCTTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTA624220.1204447948204146TruSeq Adapter, Index 22 (100% over 37bp)
CATGACTGTCATGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTA594160.11464464337973396TruSeq Adapter, Index 22 (100% over 37bp)
CTCAGCTGCTGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTA562820.10859751276925723TruSeq Adapter, Index 22 (100% over 37bp)
ACAGACTGTCTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTA556270.10733367404881618TruSeq Adapter, Index 22 (100% over 37bp)
ACTCACTGTGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTA548270.10579005423759044TruSeq Adapter, Index 22 (100% over 37bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)