FastQCFastQC Report
Fri 16 Dec 2022
EGAF00007543753

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007543753
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51826233
Sequences flagged as poor quality0
Sequence length51
%GC34

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAAGGACCTTCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC4032980.7781734782846362Illumina Single End PCR Primer 1 (100% over 38bp)
TGTAGGACCTACAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC3216270.6205872612813669Illumina Single End PCR Primer 1 (100% over 38bp)
GAAGTCAGACTTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC1579470.30476264790458535Illumina Single End PCR Primer 1 (100% over 38bp)
GTGAGGACCTCACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC1332710.2571496948273281Illumina Single End PCR Primer 1 (100% over 38bp)
CCATAGACTATGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC1295980.250062550369038Illumina Single End PCR Primer 1 (100% over 38bp)
CGGTAGACTACCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC1286000.24813688465453393Illumina Single End PCR Primer 1 (100% over 38bp)
TGCTGCAGCAGCAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC1208970.2332737553971943Illumina Single End PCR Primer 1 (100% over 38bp)
ATCAAGACTTGATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC664660.1282477929661606Illumina Single End PCR Primer 1 (100% over 38bp)
CAAGAGACTCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC641370.12375392979072973Illumina Single End PCR Primer 1 (100% over 38bp)
CATGACAGTCATGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC599410.11565764388085086Illumina Single End PCR Primer 1 (100% over 38bp)
CTCAGCAGCTGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC582980.11248743469354602Illumina Single End PCR Primer 1 (100% over 38bp)
ACAGACAGTCTGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC576370.11121201882452077Illumina Single End PCR Primer 1 (100% over 38bp)
TGACAGACTGTCAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC574930.11093416725850017Illumina Single End PCR Primer 1 (100% over 38bp)
ACTCACAGTGAGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC568300.10965489233994684Illumina Single End PCR Primer 1 (100% over 38bp)
GTTGAGACTCAACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC528700.10201397427437955Illumina Single End PCR Primer 1 (100% over 38bp)
AGAAGGTTCAGACGTGTGCTCTTCCGATCTAGAAGGACCTTCTAGATCGGA520550.10044141159169336Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)