FastQCFastQC Report
Fri 16 Dec 2022
EGAF00007543754

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007543754
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40464888
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAAGGTCCTTCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGC2424090.5990601036632055TruSeq Adapter, Index 13 (97% over 37bp)
TGTAGGTCCTACAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGC1365480.337448110569341TruSeq Adapter, Index 13 (97% over 37bp)
CGGTAGTCTACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGC847310.20939388241974127TruSeq Adapter, Index 13 (97% over 37bp)
ACTCACTGTGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGC603630.14917377258031703TruSeq Adapter, Index 13 (97% over 37bp)
GAAGTCTGACTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGC594850.14700399022480923TruSeq Adapter, Index 13 (97% over 37bp)
CCATAGTCTATGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGC587900.1452864517998913TruSeq Adapter, Index 13 (97% over 37bp)
CAAGAGTCTCTTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGC427620.1056768030594821TruSeq Adapter, Index 13 (97% over 37bp)
CATGACTGTCATGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGC416600.10295345436270575TruSeq Adapter, Index 13 (97% over 37bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)