FastQCFastQC Report
Fri 16 Dec 2022
EGAF00007543757

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007543757
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41823293
Sequences flagged as poor quality0
Sequence length51
%GC34

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTAGGACCTACAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC2338490.5591357906705242Illumina Single End PCR Primer 1 (100% over 38bp)
AGAAGGACCTTCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC1020830.24408168911998393Illumina Single End PCR Primer 1 (100% over 38bp)
GTGAGGACCTCACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC894050.21376843760246234Illumina Single End PCR Primer 1 (100% over 38bp)
CCATAGACTATGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC657920.15730946867335388Illumina Single End PCR Primer 1 (100% over 38bp)
TGCTGCAGCAGCAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC521920.12479170399136194Illumina Single End PCR Primer 1 (100% over 38bp)
GAAGTCAGACTTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC485630.11611472104790983Illumina Single End PCR Primer 1 (100% over 38bp)
CAAGAGACTCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC432360.10337779954342667Illumina Single End PCR Primer 1 (100% over 38bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)