FastQCFastQC Report
Tue 7 May 2024
EGAF00007543774

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007543774
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences115906
Total Bases5.9 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTACATGGGGGGTATCGACGATTACAAAAAAAAAAAAAAAAAAAAAAA41613.5899780856901287No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40313.477818232015599No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17161.4805100685037875No Hit
GAGTACATGGGGGTATCGACGATTACAAAAAAAAAAAAAAAAAAAAAAAA13741.1854433765292565No Hit
AGTACATGGGGGGTATCGACGATTACAAAAAAAAAAAAAAAAAAAAAAAA8620.7437061066726486No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA5010.4322468206995324No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC3550.306282677341984No Hit
GGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA3230.27867409797594606No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC3070.26486980829292706No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA3030.2614187358721723No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC2850.24588890997877588No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2460.2122409538764171No Hit
CTTTCCGCGGGGAGATTGCATCATCACAAACATTTCAACTTCGCTGAGTC2370.2044760409297189No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCC2270.19584835987783203No Hit
GTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA2150.1854951426155678No Hit
GAGTACATGGGGTCTTTCCGTCTTTCCGCGGGGAGATTGCATCATCACAA2150.1854951426155678No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAT2010.17341638914292618No Hit
AGTACATGGGGGTATCGACGATTACAAAAAAAAAAAAAAAAAAAAAAAAA1980.17082808482736012No Hit
GTACATGGGGGGTATCGACGATTACAAAAAAAAAAAAAAAAAAAAAAAAA1780.15357272272358635No Hit
GCATGAACGGGTTGCAAAAAATCGGTGGCTGCGACTGTTTATTAAAAACA1770.15270995461839765No Hit
GGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA1700.14667057788207685No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAT1560.13459182440943523No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA1430.1233758390419823No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC1390.11992476662122756No Hit
CCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAATGG1370.11819923041085018No Hit
ACCTTAGGACCGTTATTGTTACGGCCGCCGTTTACTGGGACTTCAATCAA1330.11474815799009541No Hit
GCAAATTGACACCGTAACTTCGGAAGAAGGTGTGCCCTATTAGTGTGAAG1310.11302262177971804No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCC1270.10957154935896331No Hit
GTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA1240.10698324504339723No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC1190.10266940451745381No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1160.10008110020188773No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)