FastQCFastQC Report
Thu 15 Dec 2022
EGAF00007543774

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007543774
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences115906
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTACATGGGGGGTATCGACGATTACAAAAAAAAAAAAAAAAAAAAAAAA41603.5891153175849393No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33462.8868220799613478No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14891.2846617086259555No Hit
GAGTACATGGGGGTATCGACGATTACAAAAAAAAAAAAAAAAAAAAAAAAA13731.1845806084240678No Hit
AGTACATGGGGGGTATCGACGATTACAAAAAAAAAAAAAAAAAAAAAAAAA8620.7437061066726486No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA4310.3718530533363243No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAT3330.28730177902783294No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC3190.27522302555519124No Hit
GGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA3140.2709091850292478No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCC2440.21051541766603973No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC2380.20533880903490762No Hit
CTTTCCGCGGGGAGATTGCATCATCACAAACATTTCAACTTCGCTGAGTCT2320.20016220040377547No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2190.18894621503632253No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAT2140.1846323745103791No Hit
GAGTACATGGGGTCTTTCCGTCTTTCCGCGGGGAGATTGCATCATCACAAA2100.18118130208962435No Hit
GTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA2040.1760046934584922No Hit
AGTACATGGGGGTATCGACGATTACAAAAAAAAAAAAAAAAAAAAAAAAAA1980.17082808482736012No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC1930.16651424430141668No Hit
GCATGAACGGGTTGCAAAAAATCGGTGGCTGCGACTGTTTATTAAAAACAC1770.15270995461839765No Hit
GTACATGGGGGGTATCGACGATTACAAAAAAAAAAAAAAAAAAAAAAAAAA1770.15270995461839765No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATT1750.1509844184080203No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA1660.14321950546132212No Hit
GGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA1650.1423567373561334No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA1570.1354545925146239No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCCC1560.13459182440943523No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATT1380.11906199851603885No Hit
ACCTTAGGACCGTTATTGTTACGGCCGCCGTTTACTGGGACTTCAATCAAG1330.11474815799009541No Hit
GCAAATTGACACCGTAACTTCGGAAGAAGGTGTGCCCTATTAGTGTGAAGT1310.11302262177971804No Hit
CCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAATGGC1240.10698324504339723No Hit
GTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1170.10094386830707643No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)