FastQCFastQC Report
Thu 15 Dec 2022
EGAF00007543786

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007543786
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences266620
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93563.5091140949666193No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42271.5854024454279498No Hit
GAGTACATGGGTACGAGCACGAGCGGCATCTCCGCGTGGCCCTCTCCCGGA21150.7932638211687045No Hit
GACAGAATACGGGAATCTCGTTAATCCATTCATGCGCGTCACTAATTAGAT10380.38931813067286775No Hit
GAGTACATGGGGGGTATCGACGATTACAAAAAAAAAAAAAAAAAAAAAAAA9650.36193833920936164No Hit
GGAAGTGCGTCGCTCTTCGCTAACCATACCCTATCTCGCTGCGAAAGCATT9580.3593128797539569No Hit
GCCCTCTCCCGGATTTTCATGGTCCGACGGGTAGATCCCAACGACGCTGGA9450.35443702647963393No Hit
GGTATCAACGCAGAGTACATGGGTACGAGCACGAGCGGCATCTCCGCGTGG8510.31918085664991375No Hit
CTCAAAGATGCGGGCTCTCGTTTACGAGCGAAAGCTCGTCGGGGTAACCCG8090.30342809991748554No Hit
GCTGTTTAAGTGAGCTTAAGCGAATTTCATCGCGTTGACATTCAACTCACT7810.2929262620958668No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA7670.2876753431850574No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7340.2752981771810067No Hit
GTATCAACGCAGAGTACATGGGTACGAGCACGAGCGGCATCTCCGCGTGGC7120.2670467331783062No Hit
GATTTTCATGGTCCGACGGGTAGATCCCAACGACGCTGGAAGTGCGTCGCT7120.2670467331783062No Hit
GCGGCATCTCCGCGTGGCCCTCTCCCGGATTTTCATGGTCCGACGGGTAGA6550.24566799189858227No Hit
AGTACATGGGTACGAGCACGAGCGGCATCTCCGCGTGGCCCTCTCCCGGAT6170.23141549771209963No Hit
GAGACGCCGTCTGGTGATCCCAGTAGAGTTTTCTTTTCTGTATGAGCTAAC5760.21603780661615782No Hit
ATCTCGCTGCGAAAGCATTCCAGGGAACACGTTAGCTCATACAGAAAAGAA5160.19353386842697473No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA5130.19240867151751556No Hit
GAGTACATGGGACAGAATACGGGAATCTCGTTAATCCATTCATGCGCGTCA4900.18378216187832871No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG4850.18190683369589677No Hit
ACCCTATCTCGCTGCGAAAGCATTCCAGGGAACACGTTAGCTCATACAGAA4580.1717800615107644No Hit
GCTGGAAGTGCGTCGCTCTTCGCTAACCATACCCTATCTCGCTGCGAAAGC4390.16465381441752308No Hit
CCTATCTCGCTGCGAAAGCATTCCAGGGAACACGTTAGCTCATACAGAAAA4350.16315355187157754No Hit
GCAGACGTTTATCCAGAGTCACCAAGAACCGTTTAGGAGAGCCGGCCGCTA4190.15715250168779538No Hit
CCCGGAGACGCCGTCTGGTGATCCCAGTAGAGTTTTCTTTTCTGTATGAGC4040.15152651714049958No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC3870.14515040132023105No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA3650.13689895731753057No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3590.13464856349861226No Hit
GAGACGCCGTCTGGTGATCCCAGAAGAGTTTTCTTTTCTGTATGAGCTAAC3570.1338984322256395No Hit
GAGTACATGGGGGGGTAACCCGAAAGAACCCGGAGACGCCGTCTGGTGATC3340.12527192258645264No Hit
CCTCGTTGGCGGGACACAATTTCAAGTCCCGGTCCCAAGCACGCAGGAGGT3330.12489685694996626No Hit
GGCCTCAGCCAGGTGTTGACGCAATGTGATTTCTGCTCAGTGAGTTGAATG3180.11927087240267047No Hit
CTTAAACAGCGGGAGTAACTATGACTCTCTTAAGGTAGCCAAATGCCTCGT3140.11777060985672494No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG3120.11702047858375215No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA3080.11552021603780663No Hit
GTAGAGTTTTCTTTTCTGTATGAGCTAACGTGTTCCCTGGAATGCTTTCGC2950.11064436276348362No Hit
GGAGACGCCGTCTGGTGATCCCAGTAGAGTTTTCTTTTCTGTATGAGCTAA2920.10951916585402446No Hit
GAGTACATGGGGGGCTCTCGTTTACGAGCGAAAGCTCGTCGGGGTAACCCG2910.10914410021753806No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT2880.10801890330807891No Hit
CTCGTTTACGAGCGAAAGCTCGTCGGGGTAACCCGAAAGAACCCGGAGACG2860.10726877203510615No Hit
GAAAGAACCCGGAGACGCCGTCTGGTGATCCCAGTAGAGTTTTCTTTTCTG2800.10501837821618784No Hit
TCTCAAAGATGCGGGCTCTCGTTTACGAGCGAAAGCTCGTCGGGGTAACCC2770.10389318130672867No Hit
GGAATGCTTTCGCAGCGAGATAGGGTATGGTTAGCGAAGAGCGACGCACTT2770.10389318130672867No Hit
GCGAGATAGGGTATGGTTAGCGAAGAGCGACGCACTTCCAGCGTCGTTGGG2760.1035181156702423No Hit
GAGTACATGGGGGGAATCCGACTGTCTAATTAAAACAAAGCATTGCGATGG2750.1031430500337559No Hit
GGCATTTGGCTACCTTAAGAGAGTCATAGTTACTCCCGCTGTTTAAGTGAG2670.10014252494186483No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)