FastQCFastQC Report
Thu 15 Dec 2022
EGAF00007543867

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007543867
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences91909
Sequences flagged as poor quality0
Sequence length51
%GC36

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26502.8832867292648166No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA8550.9302679824609124No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8530.9280919170048635No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA7240.7877356950897083No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC6540.7115734041279962No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCC5820.6332350477102351No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAC5070.5516325931084006No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC4750.516815545811618No Hit
GAGTGAATGGGGCGGTTCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4700.5113753821714957No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCCC3860.41998063301744115No Hit
GTGAATGGGGAGCGGTTCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3650.3971319457289275No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC3580.3895157166327563No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAACC3540.38516358572065845No Hit
GAGTGAATGGGGCGGTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3530.384075552992634No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA3140.3416422765996801No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAC3050.33184998204746No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA3030.32967391659141104No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCC3020.3285858838633866No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAACC2890.3144414583990686No Hit
GAGTGAATGGGGAGCGGTTCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2860.31117736021499526No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAC2670.29050473838253055No Hit
GAGTGAATGGGGAGCGGTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2400.26112785472587013No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCCC2360.2567757238137723No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC2320.2524235929016745No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTACC2210.24045523289340542No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTAACCC1850.20128605468452493No Hit
GTGAATGGGGAGCGGTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1800.19584589104440264No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCCCC1800.19584589104440264No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAACCC1730.1882296619482314No Hit
GTATCAACGCAGAGTGAATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA1650.17952540012403573No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA1620.17626130193996234No Hit
GGTATCAACGCAGAGTGAATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA1580.17190917102786452No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG1530.16646900738774223No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC1470.15994081101959545No Hit
AGTGAATGGGGAGCGGTTCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1450.15776474556354655No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAC1420.15450064737947317No Hit
GATCGGAAGAGCGGTTCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1410.15341261465144873Illumina Paired End Adapter 2 (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTACC1320.1436203200992286No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCCCC1250.13600409100305738No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC1250.13600409100305738No Hit
ACAGATGATAGGATGTTTCATGTGGTGTATGCATCGGGGTAGTCCGAGTAA1200.13056392736293507No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAC1190.1294758946349106No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTACCC1180.12838786190688614No Hit
ATCATAGAAGAGCTTATCACCTTTCATGATCACGCCCTCATAATCATTTTC1180.12838786190688614No Hit
GTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATTTACG1160.12621179645083724No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTACCC1100.1196836000826905No Hit
GAGTGAATGGGAGCGGTTCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1070.11641950189861712No Hit
GAGTGAATGGGCGGTTCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1060.11533146917059266No Hit
CATAATCATTTTCCTTATCTGCTTCCTAGTCCTGTATGCCCTTTTCCTAAC1020.1109793382584948No Hit
ATTGTTGATATGGACTCTAGAATAGGATTGCGCTGTTATCCCTAGGGTAAC970.10553917461837252No Hit
GCTAAGGGAGGGTAGACTGTTCAACCTGTTCCTGCTCCGGCCTCCACTATA950.10336310916232361No Hit
GAGTGAATGGGGCGGTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT940.10227507643429914No Hit
AGTGAATGGGGAGCGGTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA930.10118704370627468No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTAACCCC920.10009901097825023No Hit
CCCTTAGCAGGGAACTACTCCCACCCTGGAGCCTCCGTAGACCTAACCATC920.10009901097825023No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)