FastQCFastQC Report
Fri 16 Dec 2022
EGAF00007543878

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007543878
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60519553
Sequences flagged as poor quality0
Sequence length51
%GC36

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTAGGTCCTACAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTA4352510.7191907051924195TruSeq Adapter, Index 22 (100% over 37bp)
GTGAGGTCCTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTA1634340.27005156498759997TruSeq Adapter, Index 22 (100% over 37bp)
AGAAGGTCCTTCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTA1360330.22477528874015312TruSeq Adapter, Index 22 (100% over 37bp)
CAAGAGTCTCTTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTA757510.1251678114674773TruSeq Adapter, Index 22 (100% over 37bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)