FastQCFastQC Report
Fri 16 Dec 2022
EGAF00007543879

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007543879
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60519553
Sequences flagged as poor quality0
Sequence length51
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTAGGACCTACAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC5309160.8772635845476254Illumina Single End PCR Primer 1 (100% over 38bp)
GTGAGGACCTCACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC3135350.5180722336134902Illumina Single End PCR Primer 1 (100% over 38bp)
AGAAGGACCTTCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC1551600.2563799504599778Illumina Single End PCR Primer 1 (100% over 38bp)
CAAGAGACTCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC820880.13563880751068996Illumina Single End PCR Primer 1 (100% over 38bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN617910.1021008863036381No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)