FastQCFastQC Report
Thu 15 Dec 2022
EGAF00007543896

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007543896
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55252
Sequences flagged as poor quality0
Sequence length51
%GC35

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18963.431549989140665No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA6331.1456598856150004No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5861.0605950915803952No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA5110.9248533989719828No Hit
GAGTGAATGGGGCGGTTCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3810.6895677984507348No Hit
GTGAATGGGGAGCGGTTCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3770.6823282415116195No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAC3740.676898573807283No Hit
GAGTGAATGGGGAGCGGTTCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2800.5067689857380728No Hit
GAGTGAATGGGGAGCGGTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2540.4597118656338232No Hit
GAGTGAATGGGGCGGTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2490.4506624194599291No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA2370.4289437486425831No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC2240.4054151885904583No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA2220.40179541012090064No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAC2190.3963657424165641No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAACC2190.3963657424165641No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC2060.3728371823644393No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAC2020.365597625425324No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAACC2000.3619778469557663No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCC1790.3239701730254108No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTACC1790.3239701730254108No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC1750.31673061608629555No Hit
AGTGAATGGGGAGCGGTTCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1560.28234272062549776No Hit
GATCGGAAGAGCGGTTCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1470.26605371751248824Illumina Paired End Adapter 2 (95% over 21bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA1470.26605371751248824No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG1450.2624339390429306No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTAACCC1420.2570042713385941No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAACCC1360.24614493592992107No Hit
GTGAATGGGGAGCGGTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1340.24252515746036343No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCCC1280.23166582205169042No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGA1150.20813726199956561No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAC1130.20451748353000798No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTACC1120.20270759429522914No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAC1080.19546803735611382No Hit
GAGTGAATGGGAGCGGTTCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1060.19184825888655613No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC1010.182798812712662No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTACCC980.17736914500832549No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCCC920.1665098095996525No Hit
GAGTGAATGGGCGGTTCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA850.15384058495620068No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC820.1484109172518642No Hit
AGTGAATGGGGCGGTTCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA810.14660102801708536No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCC770.13936147107797003No Hit
AGTGAATGGGGAGCGGTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA760.1375515818431912No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTAACCCC750.13574169260841237No Hit
GAGTGAATGGGAGCGGTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA750.13574169260841237No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC740.13393180337363353No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAACC720.13031202490407587No Hit
GCTCTCAGGGAGGCTAAGAGGCGGGAGGATAGCTTGAGCCCAGGAGTTCGA710.12850213566929705No Hit
GAGTGAATGGGGCGGTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC690.12488235719973938No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTAACCCC690.12488235719973938No Hit
GATCGGAAGAGCGGTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA670.12126257873018172No Hit
GAGTGAATGGGGCGGTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT660.11945268949540289No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAC640.11583291102584521No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCA630.11402302179106638No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGAA620.11221313255628756No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA610.11040324332150873No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCCCC600.10859335408672989No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCT590.10678346485195106No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTACCC580.10497357561717223No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)