FastQCFastQC Report
Thu 15 Dec 2022
EGAF00007543913

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007543913
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences177404
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATTTACG9650.5439561678428897No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9340.5264819282541544No Hit
GCTCTCAGGGAGGCTAAGAGGCGGGAGGATAGCTTGAGCCCAGGAGTTCGA6580.3709048273996077No Hit
GATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGATTT5830.3286284412978287No Hit
ATTGTTGATATGGACTCTAGAATAGGATTGCGCTGTTATCCCTAGGGTAAC5120.2886067957881446No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAACT4650.2621135938310297No Hit
GAGATAGGTAGGAGTAGCGTGGTAAGGGTGATGAGTGTGGGGAGGAATGGG4610.2598588532389349No Hit
ACAGATGATAGGATGTTTCATGTGGTGTATGCATCGGGGTAGTCCGAGTAA4290.24182092850217582No Hit
GCTAAGGGAGGGTAGACTGTTCAACCTGTTCCTGCTCCGGCCTCCACTATA4200.23674776216996235No Hit
CCCTTAGCAGGGAACTACTCCCACCCTGGAGCCTCCGTAGACCTAACCATC4100.23111091068972514No Hit
CATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATGC3900.21983720772925075No Hit
GTTAGATATGGGGAGTAGTGTGATTGAGGTGGAGTAGATTAGGCGTAGGTA3780.21307298595296612No Hit
CCTTATTCGAGCCGAGCTGGGCCAGCCAGGCAACCTTCTAGGTAACGACCA3630.20461770873261032No Hit
ACAATATGGGAGATTATTCCGAAGCCTGGTAGGATAAGAATATAAACTTCA3560.20067191269644424No Hit
CCTCCGGCGGGGTCGAAGAAGGTGGTGTTGAGGTTGCGGTCTGTTAGTAGT3530.1989808572523731No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3520.1984171721043494No Hit
GTAATGATGTCGGGGTTGAGGGATAGGAGGAGAATGGGGGATAGGTGTATG3510.19785348695632565No Hit
TCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTT3410.19221663547608847No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACATCGAGGTCG3310.18657978399585126No Hit
GCTATCACCAGGCTCGGTAGGTTTGTCGCCTCTACCTATAAATCTTCCCAC3100.17474239588735316No Hit
TCATAATCGGAGGCTTTGGCAACTGACTAGTTCCCCTAATAATCGGTGCCC3090.17417871073932945No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCC2980.16797817411106852No Hit
CCTGGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAA2970.1674144889630448No Hit
ATCATAGAAGAGCTTATCACCTTTCATGATCACGCCCTCATAATCATTTTC2890.16290500777885505No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC2840.16008658203873646No Hit
ACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTGTC2810.1583955265946653No Hit
GGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGCC2800.15783184144664156No Hit
CTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTTG2730.15388604541047554No Hit
CCTATGATGGCAAATACAGCTCCTATTGATAGGACATAGTGGAAGTGAGCT2710.1527586751144281No Hit
GGTAGGGTGGGGGGCATCCATATAGTCACTCCAGGTTTATGGAGGGTTCTT2690.15163130481838064No Hit
GGTAGGATAAGAATATAAACTTCAGGGTGACCGAAAAATCAGAATAGGTGT2670.1505039345223332No Hit
GGGGATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATTCTAT2630.14824919393023833No Hit
CCTCATACTAGGCCTACTAACCAACACACTAACCATATACCAATGATGGCG2530.14261234245000115No Hit
GCTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGATCTGAGTT2520.1420486573019774No Hit
GCCATCATAGGAGGCTTCATTCACTGATTTCCCCTATTCTCAGGCTACACC2500.14092128700592998No Hit
GCCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCTCTCAGGGAGGCTAAGA2490.14035760185790624No Hit
AGGGATAACAGCGCAATCCTATTCTAGAGTCCATATCAACAATAGGGTTTA2410.1358481206737165No Hit
ACTATGCTTAGCCCTAAACCTCAACAGTTAAATCAACAAAACTGCTCGCCA2370.1335933800816216No Hit
CCTCTAGAGGGATATGAAGCACCGCCAGGTCCTTTGAGTTTTAAGCTGTGG2370.1335933800816216No Hit
CCACTATAGCAGATGCGAGCAGGAGTAGGAGAGAGGGAGGTAAGAGTCAGA2310.13021126919347928No Hit
CCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCAT2280.12852021374940814No Hit
GGTAGAGGGGGTGCTATAGGGTAAATACGGGTCCTATTTCAAAGATTTTTA2260.1273928434533607No Hit
CTCATCATCGAGGCTATATATTAATAGACATGGTATTAAGCCCACACGAAA2230.12570178800928952No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCCC2220.1251381028612658No Hit
GCTATAGTGGAGGCCGGAGCAGGAACAGGTTGAACAGTCTACCCTCCCTTA2210.1245744177132421No Hit
GGTTTATGGAGGGTTCTTCTACTATTAGGACTTTTCGCTTCGAAGCGAAGG2180.12288336226917093No Hit
ACTACTATCTCGCACCTGAAACAAGCTAACATGACTAACACCCTTAATTCC2140.12062862167707607No Hit
CCTAATTGGGGGGTAGGGGCTAGGCTGGAGTGGTAAAAGGCTCAGAAAAAT2130.12006493652905234No Hit
CCCATATTGTAACTTACTACTCCGGAAAAAAAGAACCATTTGGATACATAG2120.1195012513810286No Hit
CCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAA2110.1189375662330049No Hit
AGGAAGCCAATTGATATCATAGCTCAGACCATACCTATGTATCCAAATGGT2100.11837388108498118No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC2030.11442808504881513No Hit
TCTTAATTGGTGGCTGCTTTTAGGCCTACTATGGGTGTTAAATTTTTTACT2000.11273702960474398No Hit
CCTACTTACGGGACTCAACATACTAGTCACAGCCCTATACTCCCTCTACAT1990.11217334445672025No Hit
GCTTATGCGGAGGAGAATGTTTTCATGTTACTTATACTAACATTAGTTCTT1980.11160965930869654No Hit
CCTAGGGAGAGGAGGGTGGATGGAATTAAGGGTGTTAGTCATGTTAGCTTG1980.11160965930869654No Hit
CATAATCATTTTCCTTATCTGCTTCCTAGTCCTGTATGCCCTTTTCCTAAC1940.10935491871660165No Hit
CCGATTATGATGGGTATTACTATGAAGAAGATTATTACAAATGCATGGGCT1940.10935491871660165No Hit
ACTACGAAAGTGGCTTTAACATATCTGAACACACAATAGCTAAGACCCAAA1930.10879123356857795No Hit
CCTCTTCGTCTGATCCGTCCTAATCACAGCAGTCCTACTTCTCCTATCTCT1920.10822754842055421No Hit
CCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGCACACCCGTCTAT1920.10822754842055421No Hit
GATAGTAGTAGGGTCGTGGTGCTGGAGTTTAAGTTGAGTAGTAGGAATGCG1910.10766386327253048No Hit
GATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAGACATTGGAAC1890.10653649297648306No Hit
GGTATAGAATGGGGTCTCCTCCTCCGGCGGGGTCGAAGAAGGTGGTGTTGA1860.10484543753241189No Hit
GCTAAATCCCCTAGAAGTCCCACTCCTAAACACATCCGTATTACTCGCATC1810.10202701179229329No Hit
CCAATTAAGAAAGCGTTCAAGCTCAACACCCACTACCTAAAAAATCCCAAA1790.10089964149624586No Hit
AGTATCAGGCGGCGGCTTCGAAGCCAAAGTGATGTTTGGATGTAAAGTGAA1780.10033595634822212No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)