FastQCFastQC Report
Fri 16 Dec 2022
EGAF00007543930

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007543930
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55538636
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAAGGTCCTTCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCT2744400.4941424920842492TruSeq Adapter, Index 6 (97% over 36bp)
TGTAGGTCCTACAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCT2029410.36540508484940104TruSeq Adapter, Index 6 (97% over 36bp)
CCATAGTCTATGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCT1606190.2892022771319051TruSeq Adapter, Index 6 (97% over 36bp)
ATCAAGTCTTGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCT1231560.22174833389858548TruSeq Adapter, Index 6 (97% over 36bp)
GTGAGGTCCTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCT1116760.20107803871884788TruSeq Adapter, Index 6 (97% over 36bp)
TATCTCTGAGATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCT633490.11406293809592298TruSeq Adapter, Index 6 (97% over 36bp)

[OK]Adapter Content

Adapter graph