FastQCFastQC Report
Thu 15 Dec 2022
EGAF00007543940

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007543940
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73139
Sequences flagged as poor quality0
Sequence length51
%GC30

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38685.288560138913575No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA14021.916897961415934No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12501.70907450197569No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA12331.6858310887488206No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAC8681.1867813341719193No Hit
GAGTGAATGGGGCGGTTCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8151.11431657528815No Hit
GTGAATGGGGAGCGGTTCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7431.0158738839743502No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAACC6350.8682098470036506No Hit
GAGTGAATGGGGCGGTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5740.784807011307237No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA5720.7820724921040758No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA5600.7656653768851092No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAC5390.7369529252519176No Hit
GAGTGAATGGGGAGCGGTTCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5150.7041386948139844No Hit
GAGTGAATGGGGAGCGGTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4480.6125323015080874No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAC4430.6056960035001846No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAACC4160.5687799942575097No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTAACCC3460.47307182214687105No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC3240.4429921109120989No Hit
GTGAATGGGGAGCGGTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3140.4293195148962934No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA3030.4142796592789073No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTACC2830.38693446724729624No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAACCC2590.354120236809363No Hit
AGTGAATGGGGAGCGGTTCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2570.35138571760620185No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC2330.3185714871682686No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAC2300.314469708363527No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC2200.30079711234772144No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAC2090.2857572567303354No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTACC2070.2830227375271743No Hit
AGTGAATGGGGCGGTTCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1950.2666156223082077No Hit
GATCGGAAGAGCGGTTCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1850.2529430262924022Illumina Paired End Adapter 2 (95% over 21bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCC1820.24884124748766048No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTACCC1790.24473946868291885No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTACCC1790.24473946868291885No Hit
GAGTGAATGGGAGCGGTTCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1780.24337220908133825No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG1680.22969961306553277No Hit
AGTGAATGGGGAGCGGTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1660.22696509386237163No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTAACCCC1630.22286331505763No Hit
GAGTGAATGGGCGGTTCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1600.21876153625288833No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAACC1540.210557978643405No Hit
GAGTGAATGGGAGCGGTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1540.210557978643405No Hit
GTGAATGGGGCGGTTCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1520.2078234594402439No Hit
GAGTGAATGGGGCGGTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT1510.20645619983866337No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGA1500.20508894023708282No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTAACCCC1500.20508894023708282No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAC1400.1914163442212773No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA1330.18184552701021342No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC1330.18184552701021342No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1330.18184552701021342No Hit
GAGTGAATGGGGCGGTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC1270.17364196940073012No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCC1250.170907450197569No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCCC1230.16817293099440792No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAACC1200.16407115218966625No Hit
GAGTGAATGGGGCGGTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATA1140.15586759458018296No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAACCC1130.15450033497860238No Hit
GAGTGAATGGGGCGGTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAC1080.14766403697069963No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC1050.14356225816595797No Hit
GATCGGAAGAGCGGTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1000.13672596015805522No Hit
GTGAATGGGGAGCGGTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT970.13262418135331355No Hit
GAGTGAATGGGCGGTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA930.12715514294699135No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAACCCC920.12578788334541077No Hit
AGTGAATGGGGCGGTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA920.12578788334541077No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC910.12442062374383024No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCCC890.12168610454066912No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTACCCC850.11621706613434693No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCA830.11348254693118581No Hit
GAGTGAATGGGGCGGTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACC800.10938076812644416No Hit
GAGTGAATGGGGCGGTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT800.10938076812644416No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAACC800.10938076812644416No Hit
GAGTGAATGGGGCGGTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATC770.1052789893217025No Hit
GAGTGAATGGGGGAAGAGCGGTTCAAAAAAAAAAAAAAAAAAAAAAAAAAA750.10254447011854141No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTAAACCC750.10254447011854141No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTACCCC740.10117721051696085No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)