FastQCFastQC Report
Thu 15 Dec 2022
EGAF00007543943

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007543943
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22076
Sequences flagged as poor quality0
Sequence length51
%GC34

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCC5612.541221235731111No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC5562.5185722051096215No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4201.9025185722051097No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC3951.7892734190976627No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCCC3691.6714984598659177No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCC2591.1732197861931508No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCCC2481.1233919188258743No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC2231.010146765718427No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC1870.8470737452437036No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCCCC1570.7111795615147671No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1550.7021199492661714No Hit
GGTATCAACGCAGAGTGAATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA1420.6432324696502989No Hit
GTATCAACGCAGAGTGAATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA1230.5571661532886393No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCCCC1170.529987316542852No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC1050.4756296430512774No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCCCT910.41221235731110706No Hit
GTATCAACGCAGAGTGAATGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAA670.30349701032795795No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC640.2899075919550643No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCCT610.2763181735821707No Hit
GGTATCAACGCAGAGTGAATGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAA610.2763181735821707No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTCCCCCCT580.262728755209277No Hit
GTATCAACGCAGAGTGAATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA580.262728755209277No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCCCT570.25819894908497915No Hit
GGTATCAACGCAGAGTGAATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA520.23554991846348977No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCCC420.19025185722051097No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCCTT400.1811922449719152No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCCCTT400.1811922449719152No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA390.17666243884761731No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTCCCCCTT390.17666243884761731No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCCCCT380.17213263272331944No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAA310.1404239898532343No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA310.1404239898532343No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCCT290.1313643776046385No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTCCCCCCC290.1313643776046385No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCCCC280.12683457148034066No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCCCCC270.12230476535604276No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT270.12230476535604276No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCCTT260.11777495923174489No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCCCT260.11777495923174489No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTCCCCCCTT260.11777495923174489No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTACC260.11777495923174489No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCCCTT240.10871534698314912No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCC240.10871534698314912No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTCCCCCCTT230.10418554085885123No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCCTT230.10418554085885123No Hit
CTCCGAGCCCACGAGACCGATCAGTAATCTCGTATGCCGTCTTCTGCTTGA230.10418554085885123TruSeq Adapter, Index 5 (96% over 29bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)