FastQCFastQC Report
Fri 16 Dec 2022
EGAF00007543953

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007543953
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65952919
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTAGGTCCTACAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAAG7113751.0786103341385087TruSeq Adapter, Index 3 (97% over 37bp)
CAAGAGTCTCTTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAAG3150030.4776179807902058TruSeq Adapter, Index 3 (97% over 37bp)
AGAAGGTCCTTCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAAG2992940.4537994747434909TruSeq Adapter, Index 3 (97% over 37bp)
GTGAGGTCCTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAAG2479490.37594848531268193TruSeq Adapter, Index 3 (97% over 37bp)
CATGACTGTCATGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAAG1528610.23177291061218988TruSeq Adapter, Index 3 (97% over 37bp)
ATCGACTGTCGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAAG1509820.22892390858394002TruSeq Adapter, Index 3 (97% over 37bp)
CCATAGTCTATGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAAG1378650.2090354787784298TruSeq Adapter, Index 3 (97% over 37bp)
CTATTGTCAATAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAAG1256980.19058747043478091TruSeq Adapter, Index 3 (97% over 37bp)
GTGAGGTCCTTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAAG790740.11989461755286374TruSeq Adapter, Index 3 (97% over 37bp)
CGGTAGTCTACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAAG784980.11902126727704046TruSeq Adapter, Index 3 (97% over 37bp)
CTCAGCTGCTGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAAG776400.11772033926201203TruSeq Adapter, Index 3 (97% over 37bp)
ACTCACTGTGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAAG769890.1167332715023576TruSeq Adapter, Index 3 (97% over 37bp)
GTGAGGTCCTCATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAAG759570.11516851892484092TruSeq Adapter, Index 3 (97% over 37bp)
ATCAAGTCTTGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAAG664310.10072488224516643TruSeq Adapter, Index 3 (97% over 37bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)