FastQCFastQC Report
Thu 15 Dec 2022
EGAF00007544063

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007544063
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19208
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATTTACG3011.5670553935860059No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1590.8277800916284881No Hit
GCTATCACCAGGCTCGGTAGGTTTGTCGCCTCTACCTATAAATCTTCCCAC990.5154102457309454No Hit
GATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGATTT860.44773011245314454No Hit
CCTTATTCGAGCCGAGCTGGGCCAGCCAGGCAACCTTCTAGGTAACGACCA850.44252394835485215No Hit
GCTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGATCTGAGTT770.4008746355685131No Hit
ATTGTTGATATGGACTCTAGAATAGGATTGCGCTGTTATCCCTAGGGTAAC770.4008746355685131No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAACT750.39046230737192833No Hit
CCTATGATGGCAAATACAGCTCCTATTGATAGGACATAGTGGAAGTGGGCT690.35922532278217406No Hit
GCTAAGGGAGGGTAGACTGTTCAACCTGTTCCTGCTCCGGCCTCCACTATA690.35922532278217406No Hit
GTAATGATGTCGGGGTTGAGGGATAGGAGGAGAATGGGGGATAGGTGTATG680.3540191586838817No Hit
CCCTTAGCAGGGAACTACTCCCACCCTGGAGCCTCCGTAGACCTAACCATC670.34881299458558934No Hit
GGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGCC670.34881299458558934No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT620.32278217409412746No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCC610.3175760099958351No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC520.27072053311120364No Hit
TCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTT500.26030820491461887No Hit
TTATTTGCAGGGGAGCAGGAATTTAATCAAAAAGGCCAAATCCCATGTCAT480.24989587671803418No Hit
TCATAATCGGAGGCTTTGGCAACTGACTAGTTCCCCTAATAATCGGTGCCC480.24989587671803418No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACATCGAGGTCG470.2446897126197418No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC470.2446897126197418No Hit
GAGTGAATGGGGCTTTTCCTCCCTGTCGCCACCGAGGTCGCACGCGTGAGA450.23427738442315704No Hit
CTTCCACAGGAGGCCTACACGCCGCCGCTTGTGCTGCAGCCATGTCTCTAG440.22907122032486466No Hit
CATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATGC430.22386505622657227No Hit
ACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTGTC430.22386505622657227No Hit
GGTAGAGGGGGTGTTATAGGGTAAATACGGGCCCTATTTCAAAGATTTTTA420.21865889212827988No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTGACCG410.21345272802998752No Hit
CTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTTG390.20304039983340275No Hit
CCTGGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAA380.19783423573511036No Hit
CCTCTAGAGGGATATGAAGCACCGCCAGGTCCTTTGAGTTTTAAGCTGTGG380.19783423573511036No Hit
GATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATTCTATCTT370.192628071636818No Hit
GGTAATGATGTCGGGGTTGAGGGATAGGAGGAGAATGGGGGATAGGTGTAT370.192628071636818No Hit
GGGGATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATTCTAT360.1874219075385256No Hit
CCTCCGGCGGGGTCGAAGAAGGTGGTGTTGAGGTTGCGGTCTGTTAGTAGT360.1874219075385256No Hit
ACAGATGATAGGATGTTTCATGTGGTGTATGCATCGGGGTAGTCCGAGTAA350.18221574344023322No Hit
GCCATCATAGGAGGCTTCATTCACTGATTTCCCCTATTCTCAGGCTACACC350.18221574344023322No Hit
ATCATAATCGGAGGCTTTGGCAACTGACTAGTTCCCCTAATAATCGGTGCC340.17700957934194084No Hit
CCTAATTGGGGGGTAGGGGCTAGGCTGGAGTGGTAAAAGGCTCAGAAAAAT330.17180341524364848No Hit
CTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGATCTGAGTTC320.16659725114535612No Hit
GCTATAGTGGAGGCCGGAGCAGGAACAGGTTGAACAGTCTACCCTCCCTTA320.16659725114535612No Hit
CCGATTATGATGGGTATTACTATGAAGAAGATTATTACAAATGCATGGGCT320.16659725114535612No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCCC320.16659725114535612No Hit
CCACTATAGCAGATGCGAGCAGGAGTAGGAGAGAGGGAGGTAAGAGTCAGA310.16139108704706373No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC310.16139108704706373No Hit
GCGCAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCA300.15618492294877134No Hit
TCTTAATTGGTGGCTGCTTTTAGGCCTACTATGGGTGTTAAATTTTTTACT300.15618492294877134No Hit
CCAATTAGGAGGGCACTGGCCCCCAACAGGCATCACCCCGCTAAATCCCCT280.1457725947521866No Hit
GCCTTTTAGGTCATTGCATACTGAATACATAGGTATGCAAAGCGAACGCGG280.1457725947521866No Hit
GGTAGGATAAGAATATAAACTTCAGGGTGACCGAAAAATCAGAATAGGTGT270.1405664306538942No Hit
CCTGCCATAACCCAATACCAAACGCCCCTCTTCGTCTGATCCGTCCTAATC270.1405664306538942No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC260.13536026655560182No Hit
ACAATATGGGAGATTATTCCGAAGCCTGGTAGGATAAGAATATAAACTTCA250.13015410245730943No Hit
AGGGATAACAGCGCAATCCTATTCTAGAGTCCATATCAACAATAGGGTTTA250.13015410245730943No Hit
TATCAACAATAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCGATGG250.13015410245730943No Hit
CCCATATTGTAACTTACTACTCCGGAAAAAAAGAACCATTTGGATACATAG250.13015410245730943No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA240.12494793835901709No Hit
GCCCGTGAAGAGGCGGGCATAACACAGCAAGACGAGAAGACCCTATGGAGC240.12494793835901709No Hit
TCTAGAGGAGCCTGTTCTGTAATCGATAAACCCCGATCAACCTCACCACCT240.12494793835901709No Hit
GCTTTGGGGGGTTCGATTCCTTCCTTTTTTGTCTAGATTTTATGTATACGG230.1197417742607247No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCC230.1197417742607247No Hit
ACTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTCAAC230.1197417742607247No Hit
CCACTATTAACCTACTGGGAGAACTCTCTGTGCTAGTAACCACGTTCTCCT230.1197417742607247No Hit
ACTAATCACCACCCAACAATGACTAATCAAACTAACCTCAAAACAAATGAT230.1197417742607247No Hit
ACTACGAAAGTGGCTTTAACATATCTGAACACACAATAGCTAAGACCCAAA230.1197417742607247No Hit
CCTAAGATAGAGGAGACACCTGCTAGGTGTAAGGAGAAGATGGTTAGGTCT230.1197417742607247No Hit
CCCCAATAAAGCTAAAACTCACCTGAGTTGTAAAAAACTCCAGTTGACACA220.11453561016243233No Hit
GCTAGGTGTAAGGAGAAGATGGTTAGGTCTACGGAGGCTCCAGGGTGGGAG220.11453561016243233No Hit
CCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCAT220.11453561016243233No Hit
GCTTATGCGGAGGAGAATGTTTTCATGTTACTTATACTAACATTAGTTCTT220.11453561016243233No Hit
CTCCATGGGGAACTTTACTTTCATTACGCCTATAAGTTTTAACACTCATTG220.11453561016243233No Hit
CCTAAAAGGCCGGGTTTCCCCATTCGGAAATCTGCGGATCAAAGCTCGTTT220.11453561016243233No Hit
CCTACCTGCTGGCTGCCCTAGGGGGCAACTCCTCCCCCAGCGCCAAGGACA210.10932944606413994No Hit
CCTATTGATAGGACATAGTGGAAGTGGGCTACAACGTAGTACGTGTCGTGT210.10932944606413994No Hit
GATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAGACATTGGAAC200.10412328196584757No Hit
AGGAAGCCAATTGATATCATAGCTCAGACCATACCTATGTATCCAAATGGT200.10412328196584757No Hit
CCCACTATTTTGCTACATAGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTA200.10412328196584757No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)