FastQCFastQC Report
Thu 15 Dec 2022
EGAF00007544079

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007544079
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences320359
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27490.8580998192652617No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13520.42202653897658565No Hit
CCTATGATGGCAAATACAGCTCCTATTGATAGGACATAGTGGAAGTGGGCT12990.4054825992090124No Hit
ACAATATGGGAGATTATTCCGAAGCCTGGTAGGATAAGAATATAAACTTCA12560.3920601575107926No Hit
ACAGATGATAGGATGTTTCATGTGGTGTATGCATCGGGGTAGTCCGAGTAA12360.38581716137208566No Hit
CATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATGC11950.37301901928773656No Hit
CCTTATTCGAGCCGAGCTGGGCCAGCCAGGCAACCTTCTAGGTAACGACCA10780.3364974918763013No Hit
CCTCCGGCGGGGTCGAAGAAGGTGGTGTTGAGGTTGCGGTCTGTTAGTAGT10730.33493674284162456No Hit
CCCTTAGCAGGGAACTACTCCCACCCTGGAGCCTCCGTAGACCTAACCATC10420.3252600988266289No Hit
GCTAAGGGAGGGTAGACTGTTCAACCTGTTCCTGCTCCGGCCTCCACTATA10200.31839280307405127No Hit
GGTAGAGGGGGTGTTATAGGGTAAATACGGGCCCTATTTCAAAGATTTTTA9280.2896750208359996No Hit
GTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATTTACG9030.281871275662616No Hit
GCCATCATAGGAGGCTTCATTCACTGATTTCCCCTATTCTCAGGCTACACC8610.26876098377133156No Hit
GGTAGGATAAGAATATAAACTTCAGGGTGACCGAAAAATCAGAATAGGTGT7980.24909554593440486No Hit
GTAATGATGTCGGGGTTGAGGGATAGGAGGAGAATGGGGGATAGGTGTATG7920.2472226470927928No Hit
GCTATAGTGGAGGCCGGAGCAGGAACAGGTTGAACAGTCTACCCTCCCTTA7490.23380020539457294No Hit
TCATAATCGGAGGCTTTGGCAACTGACTAGTTCCCCTAATAATCGGTGCCC7340.2291179582905428No Hit
CCTAATTGGGGGGTAGGGGCTAGGCTGGAGTGGTAAAAGGCTCAGAAAAAT6670.20820392122587475No Hit
ATCATAGAAGAGCTTATCACCTTTCATGATCACGCCCTCATAATCATTTTC6570.20508242315652128No Hit
CCTCATACTAGGCCTACTAACCAACACACTAACCATATACCAATGATGGCG6530.2038338239287799No Hit
CCACTATAGCAGATGCGAGCAGGAGTAGGAGAGAGGGAGGTAAGAGTCAGA6530.2038338239287799No Hit
CCCATATTGTAACTTACTACTCCGGAAAAAAAGAACCATTTGGATACATAG6440.2010244756663618No Hit
GCTAAATCCCCTAGAAGTCCCACTCCTAAACACATCCGTATTACTCGCATC5800.18104688802249977No Hit
GGTAGGGTGGGGGGCATCCATATAGTCACTCCAGGTTTATGGAGGGTTCTT5750.17948613898782304No Hit
GGTTTATGGAGGGTTCTTCTACTATTAGGACTTTTCGCTTCGAAGCGAAGG5470.17074594439363341No Hit
CCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCAT5460.17043379458669805No Hit
GGTATAGAATGGGGTCTCCTCCTCCGGCGGGGTCGAAGAAGGTGGTGTTGA5300.16543939767573254No Hit
GCAATAATGAAGGGCAAGATGAAGTGAAAGGTAAAGAATCGTGTGAGGGTG5170.16138145018557307No Hit
GCTTTGGGGGGTTCGATTCCTTCCTTTTTTGTCTAGATTTTATGTATACGG5110.15950855134396097No Hit
CCTATTGATAGGACATAGTGGAAGTGGGCTACAACGTAGTACGTGTCGTGT5080.15857210192315496No Hit
GATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAGACATTGGAAC5080.15857210192315496No Hit
GTTAATGTGGTGGGTGAGTGAGCCCCATTGTGTTGTGGTAAATATGTAGAG4770.14889545790815928No Hit
CCTAGTTTGTTAGGGACGGATCGGAGAATTGTGTAGGCGAATAGGAAATAT4730.1476468586804179No Hit
CCTGCCATAACCCAATACCAAACGCCCCTCTTCGTCTGATCCGTCCTAATC4710.1470225590665472No Hit
CCTAAGATAGAGGAGACACCTGCTAGGTGTAAGGAGAAGATGGTTAGGTCT4640.1448375104179998No Hit
AGGAAGCCAATTGATATCATAGCTCAGACCATACCTATGTATCCAAATGGT4620.14421321080412913No Hit
GCCGGAGGAGGAGACCCCATTCTATACCAACACCTATTCTGATTTTTCGGT4560.14234031196251706No Hit
CATAATCATTTTCCTTATCTGCTTCCTAGTCCTGTATGCCCTTTTCCTAAC4470.13953096370009896No Hit
CCTCTTCGTCTGATCCGTCCTAATCACAGCAGTCCTACTTCTCCTATCTCT4420.13797021466542223No Hit
CCCTTACCATCAAATCAATTGGCCACCAATGGTACTGAACCTACGAGTACA4270.13328796756139205No Hit
CCGATTATGATGGGTATTACTATGAAGAAGATTATTACAAATGCATGGGCT4250.13266366794752138No Hit
GTTACTACGAGGGCTATGTGGCTGATTGAAGAGTATGCAATGAGCGATTTT4250.13266366794752138No Hit
GTATCAGGCGGCGGCTTCGAAGCCAAAGTGATGTTTGGATGTAAAGTGAAA4160.12985431968510328No Hit
GGTAATGATGTCGGGGTTGAGGGATAGGAGGAGAATGGGGGATAGGTGTAT4150.12954216987816794No Hit
AGTATCAGGCGGCGGCTTCGAAGCCAAAGTGATGTTTGGATGTAAAGTGAA3990.12454777296720243No Hit
TCATTATTGCAGCCCTAGCAGCACTCCACCTCCTATTCTTGCACGAAACGG3930.12267487412559036No Hit
GAGATAGGTAGGAGTAGCGTGGTAAGGGCGATGAGTGTGGGGAGGAATGGG3910.12205057451171966No Hit
GGTTAATAGTGGGGGGTAAGGCGAGGTTAGCGAGGCTTGCTAGAAGTCATC3880.12111412509091364No Hit
AGCGATGGGGGCTTCGACATGGGCTTTAGGGAGTCATAAGTGGAGTCCGTA3830.1195533760562369No Hit
TCGTAGTAACAGCCATTCTCATCCAAACCCCCTGAAGCTTCACCGGCGCAG3770.11768047721462485No Hit
CCAATTAGGAGGGCACTGGCCCCCAACAGGCATCACCCCGCTAAATCCCCT3770.11768047721462485No Hit
AGTATACCCCCGGTCGTGTAGCGGTGAAAGTGGTTTGGTTTAGACGTCCGG3700.11549542856607742No Hit
TATGGCAGGGGGTTTTATATTGATAATTGTTGTGATGAAATTGATGGCCCC3650.1139346795314007No Hit
ATCATAATCGGAGGCTTTGGCAACTGACTAGTTCCCCTAATAATCGGTGCC3480.10862813281349985No Hit
ATTCTATACCAACACCTATTCTGATTTTTCGGTCACCCTGAAGTTTATATT3470.10831598300656452No Hit
CCCATCCCTACGCATCCTTTACATAACAGACGAGGTCAACGATCCCTCCCT3360.10488233513027573No Hit
GCTTGTAGGAGGGTAAAATAGAGACCCAGTAAAATTGTAATAAGCAGTGCT3320.10363373590253436No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)