FastQCFastQC Report
Thu 15 Dec 2022
EGAF00007544080

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007544080
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences886676
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62710.7072481943799088No Hit
CATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATGC54740.6173619225060789No Hit
GTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATTTACG32720.3690186719839039No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31280.3527782414320451No Hit
ACAGATGATAGGATGTTTCATGTGGTGTATGCATCGGGGTAGTCCGAGTAA28630.3228913379859159No Hit
CCTATGATGGCAAATACAGCTCCTATTGATAGGACATAGTGGAAGTGGGCT28120.3171395188321326No Hit
ACAATATGGGAGATTATTCCGAAGCCTGGTAGGATAAGAATATAAACTTCA24820.27992186548412273No Hit
GCTAAGGGAGGGTAGACTGTTCAACCTGTTCCTGCTCCGGCCTCCACTATA24420.27541063477527306No Hit
CCTAATTGGGGGGTAGGGGCTAGGCTGGAGTGGTAAAAGGCTCAGAAAAAT23880.269320473318326No Hit
CCTCCGGCGGGGTCGAAGAAGGTGGTGTTGAGGTTGCGGTCTGTTAGTAGT23670.2669520771961799No Hit
ATTGTTGATATGGACTCTAGAATAGGATTGCGCTGTTATCCCTAGGGTAAC22350.25206501585697594No Hit
CCCATATTGTAACTTACTACTCCGGAAAAAAAGAACCATTTGGATACATAG22200.2503733043411573No Hit
CCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCAT22190.2502605235734361No Hit
GATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAGACATTGGAAC22080.2490199351285024No Hit
CCCTTAGCAGGGAACTACTCCCACCCTGGAGCCTCCGTAGACCTAACCATC21390.24123806215573673No Hit
CCTTATTCGAGCCGAGCTGGGCCAGCCAGGCAACCTTCTAGGTAACGACCA21250.23965913140763934No Hit
AGTATCAGGCGGCGGCTTCGAAGCCAAAGTGATGTTTGGATGTAAAGTGAA20930.23605014684055955No Hit
GTATCAGGCGGCGGCTTCGAAGCCAAAGTGATGTTTGGATGTAAAGTGAAA20410.230185546919055No Hit
CCTCATACTAGGCCTACTAACCAACACACTAACCATATACCAATGATGGCG20120.22691490465513897No Hit
GCTAAATCCCCTAGAAGTCCCACTCCTAAACACATCCGTATTACTCGCATC18950.21371955483175364No Hit
GGTAGGATAAGAATATAAACTTCAGGGTGACCGAAAAATCAGAATAGGTGT18820.2122534048513775No Hit
ATCATAGAAGAGCTTATCACCTTTCATGATCACGCCCTCATAATCATTTTC18770.21168950101277131No Hit
GCAATAATGAAGGGCAAGATGAAGTGAAAGGTAAAGAATCGTGTGAGGGTG16570.18687773211409803No Hit
GGTAGGGTGGGGGGCATCCATATAGTCACTCCAGGTTTATGGAGGGTTCTT16170.18236650140524838No Hit
GGTAGAGGGGGTGTTATAGGGTAAATACGGGCCCTATTTCAAAGATTTTTA15950.17988532451538106No Hit
CCTATTGATAGGACATAGTGGAAGTGGGCTACAACGTAGTACGTGTCGTGT15930.17965976297993858No Hit
CCGTATTACTCGCATCAGGAGTATCAATCACCTGAGCTCACCATAGTCTAA15740.17751692839323496No Hit
CATAATCATTTTCCTTATCTGCTTCCTAGTCCTGTATGCCCTTTTCCTAAC15410.17379516305843395No Hit
CCAATTAGGAGGGCACTGGCCCCCAACAGGCATCACCCCGCTAAATCCCCT15340.17300569768438528No Hit
GTAATGATGTCGGGGTTGAGGGATAGGAGGAGAATGGGGGATAGGTGTATG15210.17153954770400912No Hit
GCCATCATAGGAGGCTTCATTCACTGATTTCCCCTATTCTCAGGCTACACC14880.16781778236920813No Hit
CCAATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGG13940.1572163902034114No Hit
CCACTATAGCAGATGCGAGCAGGAGTAGGAGAGAGGGAGGTAAGAGTCAGA13940.1572163902034114No Hit
GCTATAGTGGAGGCCGGAGCAGGAACAGGTTGAACAGTCTACCCTCCCTTA13820.1558630209907565No Hit
CCTAGTTTGTTAGGGACGGATCGGAGAATTGTGTAGGCGAATAGGAAATAT13800.15563745945531401No Hit
CCGATTATGATGGGTATTACTATGAAGAAGATTATTACAAATGCATGGGCT13710.1546224325458228No Hit
GGTTTATGGAGGGTTCTTCTACTATTAGGACTTTTCGCTTCGAAGCGAAGG13450.15169013258507053No Hit
TCATAATCGGAGGCTTTGGCAACTGACTAGTTCCCCTAATAATCGGTGCCC13300.1499984210692519No Hit
GTTTATTGAGATGGTTTCCCACTCATCTTGACTCAGAGTGCTTTTAGTGCT13140.14819392878571203No Hit
GAGATAGGTAGGAGTAGCGTGGTAAGGGCGATGAGTGTGGGGAGGAATGGG13040.14706612110849962No Hit
CCTCTTCGTCTGATCCGTCCTAATCACAGCAGTCCTACTTCTCCTATCTCT12820.14458494421863227No Hit
GCTTTGGGGGGTTCGATTCCTTCCTTTTTTGTCTAGATTTTATGTATACGG12730.14356991730914112No Hit
GCCGGAGGAGGAGACCCCATTCTATACCAACACCTATTCTGATTTTTCGGT12720.14345713654141987No Hit
GGTATAGAATGGGGTCTCCTCCTCCGGCGGGGTCGAAGAAGGTGGTGTTGA12690.14311879423825613No Hit
AGGAAGCCAATTGATATCATAGCTCAGACCATACCTATGTATCCAAATGGT12180.1373669750844728No Hit
GCCCATGACCCCTAACAGGGGCCCTCTCAGCCCTCCTAATGACCTCCGGCC11930.13454745589144174No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACATCGAGGTCG11900.13420911358827803No Hit
GATAGTGAGGAAAGTTGAGCCAATAATGACGTGAAGTCCGTGGAAGCCTGT11620.13105125209208326No Hit
GTTAGATATGGGGAGTAGTGTGATTGAGGTGGAGTAGATTAGGCGTAGGTA11500.12969788287942832No Hit
CCGGCGTCAAAGTATTTAGCTGACTCGCCACACTCCACGGAAGCAATATGA11490.1295851021117071No Hit
GTCCTGTATGCCCTTTTCCTAACACTCACAACAAAACTAACTAATACTAAC11340.12789339059588847No Hit
CCGTAATATAGGCCTCGCCCGATGTGTAGGAAGAGGCAGATAAAGAATATT11290.12732948675728226No Hit
CCTAATGACCTCCGGCCTAGCCATGTGATTTCACTTCCACTCCATAACGCT11240.12676558291867604No Hit
CCCAATTAGGAGGGCACTGGCCCCCAACAGGCATCACCCCGCTAAATCCCC11010.12417162526108748No Hit
GCTTGTAGGAGGGTAAAATAGAGACCCAGTAAAATTGTAATAAGCAGTGCT10960.12360772142248128No Hit
CCAAATCACCACAGGACTATTCCTAGCCATGCACTACTCACCAGACGCCTC10830.12214157144210512No Hit
CCTGCCATAACCCAATACCAAACGCCCCTCTTCGTCTGATCCGTCCTAATC10790.12169044837122016No Hit
CCATTTGGATACATAGGTATGGTCTGAGCTATGATATCAATTGGCTTCCTA10570.11920927148135282No Hit
GATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGATTT10430.11763034073325544No Hit
CCTAAGATAGAGGAGACACCTGCTAGGTGTAAGGAGAAGATGGTTAGGTCT10030.11311911002440575No Hit
TCATTATTGCAGCCCTAGCAGCACTCCACCTCCTATTCTTGCACGAAACGG9920.1118785215794721No Hit
ATTCTATACCAACACCTATTCTGATTTTTCGGTCACCCTGAAGTTTATATT9790.11041237159909595No Hit
TGTAAGTAGGAGAGTGATATTTGATCAGGAGAACGTGGTTACTAGCACAGA9580.10804397547694987No Hit
CCTCAGATTCATTGAACTAGGTCTGTCCCAATGTATGGGATGGCGGATAGT9560.10781841394150739No Hit
AGGGATAACAGCGCAATCCTATTCTAGAGTCCATATCAACAATAGGGTTTA9520.10736729087062241No Hit
GTTACTACGAGGGCTATGTGGCTGATTGAAGAGTATGCAATGAGCGATTTT9500.10714172933517993No Hit
CCATATACCAATGATGGCGCGATGTAACACGAGAAAGCACATACCAAGGCC9030.10184103325228154No Hit
CCTAATAAAATTAATTAACCACTCATTCATCGACCTCCCCACCCCATCCAA9000.10150269094911783No Hit
AGGGTAACTTGTTCCGTTGGTCAAGTTATTGGATCAATTGAGTATAGTAGT8910.10048766403962664No Hit
AATGTGGGGACAGCTCATGAGTGCAAGACGTCTTGTGATGTAATTATTATA8880.10014932173646293No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)