FastQCFastQC Report
Thu 15 Dec 2022
EGAF00007544091

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007544091
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67039
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7281.0859350527305003No Hit
GTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATTTACG4230.6309759990453319No Hit
CATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATGC4210.6279926609883799No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2940.4385506943719327No Hit
ACAGATGATAGGATGTTTCATGTGGTGTATGCATCGGGGTAGTCCGAGTAA2080.310267157923No Hit
CCTTATTCGAGCCGAGCTGGGCCAGCCAGGCAACCTTCTAGGTAACGACCA2060.30728381986604814No Hit
GCTAAGGGAGGGTAGACTGTTCAACCTGTTCCTGCTCCGGCCTCCACTATA2030.3028088127806202No Hit
CCCTTAGCAGGGAACTACTCCCACCCTGGAGCCTCCGTAGACCTAACCATC1960.2923671295812885No Hit
CCTATGATGGCAAATACAGCTCCTATTGATAGGACATAGTGGAAGTGGGCT1820.27148376318262507No Hit
CCTAATTGGGGGGTAGGGGCTAGGCTGGAGTGGTAAAAGGCTCAGAAAAAT1780.26551708706872124No Hit
GATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGATTT1720.2565670728978654No Hit
CCTCATACTAGGCCTACTAACCAACACACTAACCATATACCAATGATGGCG1710.2550754038693895No Hit
CCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCAT1680.25060039678396157No Hit
TCATAATCGGAGGCTTTGGCAACTGACTAGTTCCCCTAATAATCGGTGCCC1650.24612538969853368No Hit
GGTAGAGGGGGTGTTATAGGGTAAATACGGGCCCTATTTCAAAGATTTTTA1580.23568370649920198No Hit
GCTAAATCCCCTAGAAGTCCCACTCCTAAACACATCCGTATTACTCGCATC1510.22524202329987023No Hit
AGTATCAGGCGGCGGCTTCGAAGCCAAAGTGATGTTTGGATGTAAAGTGAA1470.21927534718596636No Hit
ATTGTTGATATGGACTCTAGAATAGGATTGCGCTGTTATCCCTAGGGTAAC1460.2177836781574904No Hit
ACAATATGGGAGATTATTCCGAAGCCTGGTAGGATAAGAATATAAACTTCA1390.2073419949581587No Hit
GTATCAGGCGGCGGCTTCGAAGCCAAAGTGATGTTTGGATGTAAAGTGAAA1380.20585032592968272No Hit
CCTCCGGCGGGGTCGAAGAAGGTGGTGTTGAGGTTGCGGTCTGTTAGTAGT1340.19988364981577889No Hit
GGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGCC1300.19391697370187502No Hit
CCAATTAGGAGGGCACTGGCCCCCAACAGGCATCACCCCGCTAAATCCCCT1230.1834752905025433No Hit
GAGATAGGTAGGAGTAGCGTGGTAAGGGCGATGAGTGTGGGGAGGAATGGG1190.17750861438863946No Hit
GGTAGGATAAGAATATAAACTTCAGGGTGACCGAAAAATCAGAATAGGTGT1150.1715419382747356No Hit
ACTATGCTTAGCCCTAAACCTCAACAGTTAAATCAACAAAACTGCTCGCCA1130.16855860021778368No Hit
GATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAGACATTGGAAC1110.16557526216083174No Hit
GCTTGTAGGAGGGTAAAATAGAGACCCAGTAAAATTGTAATAAGCAGTGCT1090.16259192410387982No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAACT1080.16110025507540388No Hit
GGTTTATGGAGGGTTCTTCTACTATTAGGACTTTTCGCTTCGAAGCGAAGG1040.1551335789615No Hit
GCTATAGTGGAGGCCGGAGCAGGAACAGGTTGAACAGTCTACCCTCCCTTA1040.1551335789615No Hit
GGTCTCATTTAGGGCATCCTCTTTCTTCTTCTTGGCTTCTTCTAGGTTGCT1030.15364190993302407No Hit
CCTAATGACCTCCGGCCTAGCCATGTGATTTCACTTCCACTCCATAACGCT990.1476752338191202No Hit
GCTATCACCAGGCTCGGTAGGTTTGTCGCCTCTACCTATAAATCTTCCCAC970.1446918957621683No Hit
GGTAGGGTGGGGGGCATCCATATAGTCACTCCAGGTTTATGGAGGGTTCTT970.1446918957621683No Hit
GCCCATGACCCCTAACAGGGGCCCTCTCAGCCCTCCTAATGACCTCCGGCC970.1446918957621683No Hit
AGGAAGCCAATTGATATCATAGCTCAGACCATACCTATGTATCCAAATGGT960.14320022673369234No Hit
ACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTGTC940.1402168886767404No Hit
CCACTATAGCAGATGCGAGCAGGAGTAGGAGAGAGGGAGGTAAGAGTCAGA900.13425021256283656No Hit
CCTGCCATAACCCAATACCAAACGCCCCTCTTCGTCTGATCCGTCCTAATC900.13425021256283656No Hit
TCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTT870.12977520547740867No Hit
CTCCATGGGGAACTTTACTTTCATTACGCCTATAAGTTTTAACACTCATTG870.12977520547740867No Hit
CCGATTATGATGGGTATTACTATGAAGAAGATTATTACAAATGCATGGGCT860.1282835364489327No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACATCGAGGTCG850.12679186742045676No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC840.12530019839198078No Hit
CCCAATTAGGAGGGCACTGGCCCCCAACAGGCATCACCCCGCTAAATCCCC840.12530019839198078No Hit
CCTCTTCGTCTGATCCGTCCTAATCACAGCAGTCCTACTTCTCCTATCTCT830.12380852936350482No Hit
GCCATCATAGGAGGCTTCATTCACTGATTTCCCCTATTCTCAGGCTACACC830.12380852936350482No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC830.12380852936350482No Hit
GCTTTGGGGGGTTCGATTCCTTCCTTTTTTGTCTAGATTTTATGTATACGG820.12231686033502888No Hit
TATGGCAGGGGGTTTTATATTGATAATTGTTGTGATGAAATTGATGGCCCC820.12231686033502888No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA810.1208251913065529No Hit
ACTACGAAAGTGGCTTTAACATATCTGAACACACAATAGCTAAGACCCAAA800.11933352227807693No Hit
GCTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGATCTGAGTT790.11784185324960099No Hit
CCTAAGATAGAGGAGACACCTGCTAGGTGTAAGGAGAAGATGGTTAGGTCT790.11784185324960099No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC780.11635018422112502No Hit
CCTATTGATAGGACATAGTGGAAGTGGGCTACAACGTAGTACGTGTCGTGT760.11336684616417309No Hit
CCCATATTGTAACTTACTACTCCGGAAAAAAAGAACCATTTGGATACATAG740.11038350810722117No Hit
CCAATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGG730.1088918390787452No Hit
GATTTAGCGGGGTGATGCCTGTTGGGGGCCAGTGCCCTCCTAATTGGGGGG710.10590850102179328No Hit
ATCATAATCGGAGGCTTTGGCAACTGACTAGTTCCCCTAATAATCGGTGCC710.10590850102179328No Hit
GGTATAGAATGGGGTCTCCTCCTCCGGCGGGGTCGAAGAAGGTGGTGTTGA690.10292516296484136No Hit
GATGAAGGCTACAAAGTAAGCGCAAGTACCCACGTAAAGACGTTAGGTCAA690.10292516296484136No Hit
CTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTTG680.10143349393636539No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)