FastQCFastQC Report
Thu 15 Dec 2022
EGAF00007544134

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007544134
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2758
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATTTACG762.755620014503263No Hit
TCATAATCGGAGGCTTTGGCAACTGACTAGTTCCCCTAATAATCGGTGCCC361.305293691080493No Hit
GCTAAGGGAGGGTAGACTGTTCAACCTGTTCCTGCTCCGGCCTCCACTATA270.9789702683103698No Hit
CCTTATTCGAGCCGAGCTGGGCCAGCCAGGCAACCTTCTAGGTAACGACCA210.7614213197969544No Hit
CCTAATTGGGGGGTAGGGGCTAGGCTGGAGTGGTAAAAGGCTCAGAAAAAT190.6889050036258157No Hit
GCTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGATCTGAGTT170.6163886874546773No Hit
GGTAGAGGGGGTGTTATAGGGTAAATACGGGCCCTATTTCAAAGATTTTTA150.5438723712835388No Hit
CCTCATACTAGGCCTACTAACCAACACACTAACCATATACCAATGATGGCG140.5076142131979695No Hit
ATCATAATCGGAGGCTTTGGCAACTGACTAGTTCCCCTAATAATCGGTGCC130.47135605511240025No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACATCGAGGTCG120.43509789702683105No Hit
ACAATATGGGAGATTATTCCGAAGCCTGGTAGGATAAGAATATAAACTTCA110.3988397389412618No Hit
GCTATCACCAGGCTCGGTAGGTTTGTCGCCTCTACCTATAAATCTTCCCAC100.36258158085569253No Hit
GGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGCC100.36258158085569253No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90.3263234227701233No Hit
CTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGATCTGAGTTC90.3263234227701233No Hit
CCCTTAGCAGGGAACTACTCCCACCCTGGAGCCTCCGTAGACCTAACCATC90.3263234227701233No Hit
GTAATGATGTCGGGGTTGAGGGATAGGAGGAGAATGGGGGATAGGTGTATG90.3263234227701233No Hit
CCAATTAGGAGGGCACTGGCCCCCAACAGGCATCACCCCGCTAAATCCCCT80.290065264684554No Hit
CCTCCGGCGGGGTCGAAGAAGGTGGTGTTGAGGTTGCGGTCTGTTAGTAGT80.290065264684554No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC80.290065264684554No Hit
CCGATTATGATGGGTATTACTATGAAGAAGATTATTACAAATGCATGGGCT80.290065264684554No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC80.290065264684554No Hit
ACAGATGATAGGATGTTTCATGTGGTGTATGCATCGGGGTAGTCCGAGTAA80.290065264684554No Hit
GGTTTATGGAGGGTTCTTCTACTATTAGGACTTTTCGCTTCGAAGCGAAGG80.290065264684554No Hit
CCACTATTAACCTACTGGGAGAACTCTCTGTGCTAGTAACCACGTTCTCCT80.290065264684554No Hit
GCCATCATAGGAGGCTTCATTCACTGATTTCCCCTATTCTCAGGCTACACC80.290065264684554No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCC80.290065264684554No Hit
TACATGAGTAGGCGTTCTCCTTGTGCTCTCTCCTTCTTCTTCTCTTTCGAA70.25380710659898476No Hit
CCCATCCCTACGCATCCTTTACATAACAGACGAGGTCAACGATCCCTCCCT70.25380710659898476No Hit
CATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATGC70.25380710659898476No Hit
GGTAATGATGTCGGGGTTGAGGGATAGGAGGAGAATGGGGGATAGGTGTAT70.25380710659898476No Hit
CCTATGATGGCAAATACAGCTCCTATTGATAGGACATAGTGGAAGTGGGCT70.25380710659898476No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCC70.25380710659898476No Hit
CCTAATGACCTCCGGCCTAGCCATGTGATTTCACTTCCACTCCATAACGCT60.21754894851341552No Hit
GTTAATGTGGTGGGTGAGTGAGCCCCATTGTGTTGTGGTAAATATGTAGAG60.21754894851341552No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC60.21754894851341552No Hit
GCCCATGACCCCTAACAGGGGCCCTCTCAGCCCTCCTAATGACCTCCGGCC60.21754894851341552No Hit
GCCCGTGAAGAGGCGGGCATAACACAGCAAGACGAGAAGACCCTATGGAGC60.21754894851341552No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAACT60.21754894851341552No Hit
AGGGATAACAGCGCAATCCTATTCTAGAGTCCATATCAACAATAGGGTTTA60.21754894851341552No Hit
ACATGAGTAGGCGTTCTCCTTGTGCTCTCTCCTTCTTCTTCTCTTTCGAAC60.21754894851341552No Hit
TACTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTCAA60.21754894851341552No Hit
CTGCCATAACCCAATACCAAACGCCCCTCTTCGTCTGATCCGTCCTAATCA50.18129079042784627No Hit
GCTATCATGAGGGAGCGGGTCGGGGACCGGCTGCCAAGCTTCACAGAGCAG50.18129079042784627No Hit
CCTGTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGT50.18129079042784627No Hit
ATTGTTGATATGGACTCTAGAATAGGATTGCGCTGTTATCCCTAGGGTAAC50.18129079042784627No Hit
CATGATAGCTGGGTAATCTCCCCGTGTTAAGGGATCCATGTACCATCCAAA50.18129079042784627No Hit
GCAATTGGAGCCATTTATTCTCCTGGCTCAACGTAAAGAAAAAGAAAGAAG50.18129079042784627No Hit
GTTACTACGAGGGCTATGTGGCTGATTGAAGAGTATGCAATGAGCGATTTT50.18129079042784627No Hit
CCTAGCCTTACTACTAATAATTATTACATTTTGACTACCACAACTCAACGG50.18129079042784627No Hit
TATGGCAGGGGGTTTTATATTGATAATTGTTGTGATGAAATTGATGGCCCC50.18129079042784627No Hit
CATAATCGGAGGCTTTGGCAACTGACTAGTTCCCCTAATAATCGGTGCCCC50.18129079042784627No Hit
CCCAATTAGGAGGGCACTGGCCCCCAACAGGCATCACCCCGCTAAATCCCC50.18129079042784627No Hit
GATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGATTT50.18129079042784627No Hit
GCTTTGGGGGGTTCGATTCCTTCCTTTTTTGTCTAGATTTTATGTATACGG50.18129079042784627No Hit
TCTTAATTGGTGGCTGCTTTTAGGCCTACTATGGGTGTTAAATTTTTTACT50.18129079042784627No Hit
GGTAGGATAAGAATATAAACTTCAGGGTGACCGAAAAATCAGAATAGGTGT50.18129079042784627No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCCC50.18129079042784627No Hit
ACTATTAACCTACTGGGAGAACTCTCTGTGCTAGTAACCACGTTCTCCTGA40.145032632342277No Hit
AGGGTAACTTGTTCCGTTGGTCAAGTTATTGGATCAATTGAGTATAGTAGT40.145032632342277No Hit
CCTAAGATAGAGGAGACACCTGCTAGGTGTAAGGAGAAGATGGTTAGGTCT40.145032632342277No Hit
GCTAGGTGTAAGGAGAAGATGGTTAGGTCTACGGAGGCTCCAGGGTGGGAG40.145032632342277No Hit
CCTATTCTACACCCTAGTAGGCTCCCTTCCCCTACTCATCGCACTAATTTA40.145032632342277No Hit
CCTGGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAA40.145032632342277No Hit
CACCGGAGTCGGGTTCCGGCGGCTTAGCACAGGGCTGCAATTGTCGTCGCC40.145032632342277No Hit
GATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAGACATTGGAAC40.145032632342277No Hit
CCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATTAAAAATTTCGG40.145032632342277No Hit
GCAAACAGGCACATTTTTCTCCTCTGGGCAGCAGCAGACACATTTGTCTGA40.145032632342277No Hit
AACATACAGTAGGGTCTAGAGATCATCTACCATTATACACAGATCCAGATT40.145032632342277No Hit
ACTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTCAAC40.145032632342277No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC40.145032632342277No Hit
ATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGATCTGAGTTCAG40.145032632342277No Hit
ACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTGTC40.145032632342277No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCCC40.145032632342277No Hit
TATCAACAATAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCGATGG40.145032632342277No Hit
CCTGCCATAACCCAATACCAAACGCCCCTCTTCGTCTGATCCGTCCTAATC30.10877447425670776No Hit
CGTAGTAACAGCCATTCTCATCCAAACCCCCTGAAGCTTCACCGGCGCAGT30.10877447425670776No Hit
AACCTCCGAGCAGTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTAT30.10877447425670776No Hit
GATTCCTAGGGGGTTGTTTGATCCCGTTTCGTGCAAGAATAGGAGGTGGAG30.10877447425670776No Hit
GGTAAGGGAGGGATCGTTGACCTCGTCTGTTATGTAAAGGATGCGTAGGGA30.10877447425670776No Hit
CCTTTTTTGTCTAGATTTTATGTATACGGGTTCTTCGAATGTGTGGTAGGG30.10877447425670776No Hit
CCTCTCCCTAGGAGGCCTGCCCCCGCTAACCGGCTTTTTGCCCAAATGGGC30.10877447425670776No Hit
GATATATAGCCATGTAAAAGTGCATGGCCATGCATGCGTGCATGGCAAAAA30.10877447425670776No Hit
GAGTAATCCAGGTCGGTTTCTATCTACTTCAAATTCCTCCCTGTACGAAAG30.10877447425670776No Hit
TGTGATTGGTGGGTCATTATGTGTTGTCGTGCAGGTAGAGGCTTACTAGAA30.10877447425670776No Hit
GTAATCCAGGTCGGTTTCTATCTACTTCAAATTCCTCCCTGTACGAAAGGA30.10877447425670776No Hit
CCGATGGGGGGGCCTCCCCGCATTCTCGACCGGCCGAATCGGCGTCCCCGC30.10877447425670776No Hit
ACTAAATACTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACT30.10877447425670776No Hit
TCATAATCGGAGGCTTTGGCAACTGACTAGTTCCCCGAATAATCGGTGCCC30.10877447425670776No Hit
GGTATAGAATGGGGTCTCCTCCTCCGGCGGGGTCGAAGAAGGTGGTGTTGA30.10877447425670776No Hit
CCCTCTCTCCTACTCCTGCTCGCATCTGCTATAGTGGAGGCCGGAGCAGGA30.10877447425670776No Hit
CCTCTAGAGGGATATGAAGCACCGCCAGGTCCTTTGAGTTTTAAGCTGTGG30.10877447425670776No Hit
CCTCTTCGGGTTCCTTTTTCAACTCTGTCTCGAACTCCTTTGCAGCCTGCT30.10877447425670776No Hit
AGCGATGGGGGCTTCGACATGGGCTTTAGGGAGTCATAAGTGGAGTCCGTA30.10877447425670776No Hit
GCATATCAAGTTTGATTCCACGATGTAGTTGCATTGAACTCACACGTTACA30.10877447425670776No Hit
GTGAATGGGGAGGTCGGCATAGAGAAAGAGAAAGCAGAAAGAAGAAAAAGC30.10877447425670776No Hit
ACAACGTTGGGGCCTTTGCGTAGTTGTATATAGCCTAGAATTTTTCGTTCG30.10877447425670776No Hit
GGTGATTCCTAGGGGGTTGTTTGATCCCGTTTCGTGCAAGAATAGGAGGTG30.10877447425670776No Hit
AAATGAGACCAGGGAATCAGAGACAAAGCTGAAGGAGCTCCCAGGAGTGTG30.10877447425670776No Hit
ATCAACAATAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCGATGGT30.10877447425670776No Hit
CCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAA30.10877447425670776No Hit
CCTCACATACACATGTTTATATGTAGAATATGAGAAATTAACAACGATGAA30.10877447425670776No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)