FastQCFastQC Report
Thu 15 Dec 2022
EGAF00007544135

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007544135
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences176766
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16790.9498432956564045No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8370.4735073486982791No Hit
GTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATTTACG8320.4706787504384327No Hit
CATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATGC6580.3722435309957797No Hit
CCTATGATGGCAAATACAGCTCCTATTGATAGGACATAGTGGAAGTGGGCT5820.32924883744611516No Hit
ACAGATGATAGGATGTTTCATGTGGTGTATGCATCGGGGTAGTCCGAGTAA5360.3032257334555288No Hit
CCTTATTCGAGCCGAGCTGGGCCAGCCAGGCAACCTTCTAGGTAACGACCA5300.2998314155437132No Hit
CCCTTAGCAGGGAACTACTCCCACCCTGGAGCCTCCGTAGACCTAACCATC5050.2856884242444814No Hit
GCTAAGGGAGGGTAGACTGTTCAACCTGTTCCTGCTCCGGCCTCCACTATA5000.28285982598463505No Hit
CCTCCGGCGGGGTCGAAGAAGGTGGTGTTGAGGTTGCGGTCTGTTAGTAGT4670.26419107746964915No Hit
ATTGTTGATATGGACTCTAGAATAGGATTGCGCTGTTATCCCTAGGGTAAC4190.23703653417512416No Hit
GCCATCATAGGAGGCTTCATTCACTGATTTCCCCTATTCTCAGGCTACACC4030.22798501974361585No Hit
ACAATATGGGAGATTATTCCGAAGCCTGGTAGGATAAGAATATAAACTTCA3950.22345926252786166No Hit
CCTCATACTAGGCCTACTAACCAACACACTAACCATATACCAATGATGGCG3780.2138420284443841No Hit
TCATAATCGGAGGCTTTGGCAACTGACTAGTTCCCCTAATAATCGGTGCCC3500.19800187818924453No Hit
CCTAATTGGGGGGTAGGGGCTAGGCTGGAGTGGTAAAAGGCTCAGAAAAAT3490.19743615853727528No Hit
GATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGATTT3450.19517327992939817No Hit
GCTATGTGTCTGGGTTTCATCCATCCGACATTGAAGTTGACTTACTGAAGA3240.1832931672380435No Hit
GCTATAGTGGAGGCCGGAGCAGGAACAGGTTGAACAGTCTACCCTCCCTTA3120.17650453141441227No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAACT2970.1680187366348732No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACATCGAGGTCG2960.16745301698290396No Hit
GCTTTGGGGGGTTCGATTCCTTCCTTTTTTGTCTAGATTTTATGTATACGG2950.1668872973309347No Hit
CCTATTGATAGGACATAGTGGAAGTGGGCTACAACGTAGTACGTGTCGTGT2890.16349297941911908No Hit
CCTCTAGAGGGATATGAAGCACCGCCAGGTCCTTTGAGTTTTAAGCTGTGG2860.16179582046321125No Hit
GGTAGGATAAGAATATAAACTTCAGGGTGACCGAAAAATCAGAATAGGTGT2820.15953294185533418No Hit
CCCATATTGTAACTTACTACTCCGGAAAAAAAGAACCATTTGGATACATAG2810.1589672222033649No Hit
GATAGTTAAGTGGGATCGAGACATGTAAGCAGCATCATGGAGGTTTGAAGA2800.15840150255139562No Hit
GGTTTATGGAGGGTTCTTCTACTATTAGGACTTTTCGCTTCGAAGCGAAGG2740.15500718463958002No Hit
CCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCAT2730.15444146498761074No Hit
GCTAAATCCCCTAGAAGTCCCACTCCTAAACACATCCGTATTACTCGCATC2690.15217858637973364No Hit
ACTATGCTTAGCCCTAAACCTCAACAGTTAAATCAACAAAACTGCTCGCCA2670.15104714707579514No Hit
CCACTATAGCAGATGCGAGCAGGAGTAGGAGAGAGGGAGGTAAGAGTCAGA2640.1493499881198873No Hit
GATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAGACATTGGAAC2640.1493499881198873No Hit
CCTAGAGCTACCTGTGGAGCAACCTGCTCAGATACATCAAACATGGAGACA2560.14482423090413316No Hit
GCTATCACCAGGCTCGGTAGGTTTGTCGCCTCTACCTATAAATCTTCCCAC2510.1419956326442868No Hit
GGTAGGGTGGGGGGCATCCATATAGTCACTCCAGGTTTATGGAGGGTTCTT2430.13746987542853265No Hit
CCTAAGATAGAGGAGACACCTGCTAGGTGTAAGGAGAAGATGGTTAGGTCT2430.13746987542853265No Hit
AGGAAGCCAATTGATATCATAGCTCAGACCATACCTATGTATCCAAATGGT2400.13577271647262482No Hit
GAGTGAATGGGGTCTTTCCGTCTAACCGCGGGAAGTCTGCATCTTCACAGA2370.13407555751671701No Hit
ACATTTTGTGCATAAAGTGTAAGTGTATAAGCATATCAATATTAAAAAGCA2370.13407555751671701No Hit
ACATGAGTAGGCGTTCTCCTTGTGCTCTCTCCTTCTTCTTCTCTTTCGAAC2340.1323783985608092No Hit
GGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGCC2280.12898408064899358No Hit
AGTATCAGGCGGCGGCTTCGAAGCCAAAGTGATGTTTGGATGTAAAGTGAA2260.12785264134505503No Hit
GTAATGATGTCGGGGTTGAGGGATAGGAGGAGAATGGGGGATAGGTGTATG2200.12445832343323943No Hit
GTATCAGGCGGCGGCTTCGAAGCCAAAGTGATGTTTGGATGTAAAGTGAAA2190.12389260378127015No Hit
GGTATAGAATGGGGTCTCCTCCTCCGGCGGGGTCGAAGAAGGTGGTGTTGA2120.11993256621748527No Hit
CCGATTATGATGGGTATTACTATGAAGAAGATTATTACAAATGCATGGGCT2110.119366846565516No Hit
TACATGAGTAGGCGTTCTCCTTGTGCTCTCTCCTTCTTCTTCTCTTTCGAA2110.119366846565516No Hit
CTTAACTATCTTGGGCTGTGACAAAGTCACATGGTTCACACGGCAGGCATA2010.1137096500458233No Hit
CCAATTAGGAGGGCACTGGCCCCCAACAGGCATCACCCCGCTAAATCCCCT1990.11257821074188476No Hit
ACTACGAAAGTGGCTTTAACATATCTGAACACACAATAGCTAAGACCCAAA1950.11031533213400767No Hit
GCCGTTTACCGGGGCTTCAATTCAAGGCTTGCACCTCTCCTTTTAACCTTC1890.10692101422219205No Hit
GCCGGAGGAGGAGACCCCATTCTATACCAACACCTATTCTGATTTTTCGGT1870.10578957491825351No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1810.10239525700643788No Hit
ATCATAATCGGAGGCTTTGGCAACTGACTAGTTCCCCTAATAATCGGTGCC1790.10126381770249936No Hit
CCACATGACCGTACCCTAAACCGACACAGGTGGACTGGTAGAGAATACCAA1790.10126381770249936No Hit
CCTAATGACCTCCGGCCTAGCCATGTGATTTCACTTCCACTCCATAACGCT1770.10013237839856082No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)