FastQCFastQC Report
Fri 16 Dec 2022
EGAF00007544157

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007544157
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42950662
Sequences flagged as poor quality0
Sequence length51
%GC36

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAAGGTCCTTCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAAG1315760.3063421932821431TruSeq Adapter, Index 3 (97% over 37bp)
TGTAGGTCCTACAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAAG1167680.27186542549681775TruSeq Adapter, Index 3 (97% over 37bp)
CCATAGTCTATGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAAG490900.11429393102252998TruSeq Adapter, Index 3 (97% over 37bp)
GTGAGGTCCTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAAG458620.10677833091373538TruSeq Adapter, Index 3 (97% over 37bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)