FastQCFastQC Report
Fri 16 Dec 2022
EGAF00007544158

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007544158
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42950662
Sequences flagged as poor quality0
Sequence length51
%GC33

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAAGGACCTTCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC1318660.3070173866004673Illumina Single End PCR Primer 1 (100% over 38bp)
TGTAGGACCTACAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC1198790.27910862002546083Illumina Single End PCR Primer 1 (100% over 38bp)
GTGAGGACCTCACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC550290.12812142453124473Illumina Single End PCR Primer 1 (100% over 38bp)
CCATAGACTATGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC473520.11024742761822856Illumina Single End PCR Primer 1 (100% over 38bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)