FastQCFastQC Report
Fri 16 Dec 2022
EGAF00007544163

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007544163
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56144154
Sequences flagged as poor quality0
Sequence length51
%GC36

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTAGGTCCTACAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCT1354860.24131808985847397TruSeq Adapter, Index 6 (97% over 36bp)
AGAAGGTCCTTCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCT1006450.17926176249801537TruSeq Adapter, Index 6 (97% over 36bp)
ACTCACTGTGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCT601100.10706368467142634TruSeq Adapter, Index 6 (97% over 36bp)
CCATAGTCTATGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCT575720.10254317840464743TruSeq Adapter, Index 6 (97% over 36bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)