FastQCFastQC Report
Thu 15 Dec 2022
EGAF00007544239

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007544239
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences625088
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATCTTGAGGTGGGTTTCACACTTAGATGCTTTCAGCGTTTATCCCTTCCC102641.6420088051602333No Hit
CCCTACATAGCTACCCAGCGATGCCTTTGGCAAGACAACTGGTACACCAGC64571.0329745571823488No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57610.9216302344629876No Hit
ATCTTGAGGTGGGTTTCACACTTAGATGCTTTCAGCGTTTATCCCTTCCCT41240.659747107607249No Hit
GGTATCAACGCAGAGTACATGGGAAATCTCATCTTGAGGTGGGTTTCACAC40690.6509483464728166No Hit
TCCCTACATAGCTACCCAGCGATGCCTTTGGCAAGACAACTGGTACACCAG33210.5312851950445378No Hit
GGGGATAACAGGCTTATCTCCCCCAAGAGTTCACATCGACGGGGAGGTTTG32640.5221664789597624No Hit
GTTATCCCCAGGGTAGCTTTTATCCGTTGAGCGATGGCCCTTCCATACGGA32340.5173671547046176No Hit
GTATCAACGCAGAGTACATGGGAAATCTCATCTTGAGGTGGGTTTCACACT29890.4781726732876011No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28180.45081652503327535No Hit
CCCTCAGAATGGTTGGAAATCATTCGCAGAGTGTAAAGGTATAAGGGAGCT25140.40218337258114056No Hit
CCTAGTACGAGAGGACCAGAGTGGACTTACCGCTGGTGTACCAGTTGTCTT21750.3479510084980035No Hit
CCCAAGGGTTGGGCTGTTCGCCCATTAAAGCGGCACGCGAGCTGGGTTCAG21450.3431516842428586No Hit
ACATAGCTACCCAGCGATGCCTTTGGCAAGACAACTGGTACACCAGCGGTA20420.32667400430019455No Hit
GCGACGGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAGCTCGCGT20210.32331447732159313No Hit
ATCCGTCGCGGGCGTAGGAAATTTGAGAGGATCTGCTCCTAGTACGAGAGG19510.3121160540595884No Hit
GCATCTTCACAGGTACTAAAATTTCACCGAGTCTCTCGTTGAGACAGTGCC19110.3057169550527286No Hit
GCTCGCGTGCCGCTTTAATGGGCGAACAGCCCAACCCTTGGGACCGACTAC18760.3001177434217262No Hit
GAGTACATGGGAAATCTCATCTTGAGGTGGGTTTCACACTTAGATGCTTTC18430.29483848674106683No Hit
GATAGGTGATCCCTATCGGAGACAGTGTCTGACGGGCAGTTTGACTGGGGC17290.27660105457151635No Hit
GTGAAGATGCAGGTTACCCGCGACAGGACGGAAAGACCCCATGGAGCTTTA17210.27532123477014436No Hit
CCTAAAAGGTAACGGAGGCGCCCAAAGGTTCCCTCAGAATGGTTGGAAATC16590.2654026313095116No Hit
CCTATACATCATCTTACGATTTAGCAGAGAGCTGTGTTTTTGATAAACAGT16530.26444276645848264No Hit
GCGGTAAGTCCACTCTGGTCCTCTCGTACTAGGAGCAGATCCTCTCAAATT15920.2546841404730214No Hit
CCCAGCGATGCCTTTGGCAAGACAACTGGTACACCAGCGGTAAGTCCACTC15310.24492551448756014No Hit
TCTTGAGGTGGGTTTCACACTTAGATGCTTTCAGCGTTTATCCCTTCCCTA15010.2401261902324153No Hit
GATGTATAGGGGGTGACGCCTGCCCGGTGCTGGAAGGTTAAGAGGAGTGCT14380.23004760929661106No Hit
GAGTACATGGGGTCTTTCCGTCCTGTCGCGGGTAACCTGCATCTTCACAGG14170.22668808231800963No Hit
CCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCCGCACGAAAGGC14120.22588819494215212No Hit
CTCGTACTAGGAGCAGATCCTCTCAAATTTCCTACGCCCGCGACGGATAGG11740.18781355585133613No Hit
CCCTGGGGATAACAGGCTTATCTCCCCCAAGAGTTCACATCGACGGGGAGG11630.18605380362444968No Hit
GCACGCGAGCTGGGTTCAGAACGTCGTGAGACAGTTCGGTCCCTATCCGTC11510.18413407392239173No Hit
GGTATAAGGGAGCTTGACTGCGAGAGCTACAACTCGAGCAGGGACGAAAGT11370.1818943892699908No Hit
TCCGTCGCGGGCGTAGGAAATTTGAGAGGATCTGCTCCTAGTACGAGAGGA11330.1812544793693048No Hit
GCGATGCCTTTGGCAAGACAACTGGTACACCAGCGGTAAGTCCACTCTGGT11210.17933474966724686No Hit
CCCTATCCGTCGCGGGCGTAGGAAATTTGAGAGGATCTGCTCCTAGTACGA11100.1775749974403604No Hit
CCTATCCTGTACATGTGGTACAGACACTCAATATCAAACTGCAGTAAAGCT11000.17597522268864543No Hit
ACCTTTTAGGAGGCGACCGCCCCAGTCAAACTGCCCGTCAGACACTGTCTC10020.16029743012183884No Hit
CACATCGACGGGGAGGTTTGGCACCTCGATGTCGGCTCGTCGCATCCTGGG9930.1588576328452954No Hit
CGTACTAGGAGCAGATCCTCTCAAATTTCCTACGCCCGCGACGGATAGGGA9860.15773779051909492No Hit
ACTACAGCCCCAGGATGCGACGAGCCGACATCGAGGTGCCAAACCTCCCCG9800.15677792566806595No Hit
CCTCTCGTACTAGGAGCAGATCCTCTCAAATTTCCTACGCCCGCGACGGAT9580.15325842121429303No Hit
CATGTACAGGATAGGTAGGAGTCTACGAGATCGGGACGCCAGTTTCGAAGG9320.14909900685983413No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9300.14877905190949114No Hit
GCTTGACTGCGAGAGCTACAACTCGAGCAGGGACGAAAGTCGGGCTTAGTG9080.14525954745571823No Hit
CCCGTCGATGTGAACTCTTGGGGGAGATAAGCCTGTTATCCCCAGGGTAGC9030.14445966007986075No Hit
GACTTTCGTCCCTGCTCGAGTTGTAGCTCTCGCAGTCAAGCTCCCTTATAC8940.1430198628033173No Hit
GGGCTGTAGTCGGTCCCAAGGGTTGGGCTGTTCGCCCATTAAAGCGGCACG8830.14126011057643084No Hit
CCTGTACATGTGGTACAGACACTCAATATCAAACTGCAGTAAAGCTCCATG8750.13998029077505889No Hit
AGTAAACGGCGGCCGTAACAATAACGGTCCTAAGGTAGCGAAATTCCTTGT8710.1393403808743729No Hit
CCTGTCGCGGGTAACCTGCATCTTCACAGGTACTAAAATTTCACCGAGTCT8450.135180966519914No Hit
AGATGCTTTCAGCGTTTATCCCTTCCCTACATAGCTACCCAGCGATGCCTT8410.13454105661922802No Hit
CGTCGCATCCTGGGGCTGTAGTCGGTCCCAAGGGTTGGGCTGTTCGCCCAT8140.13022166478959762No Hit
CATGGAGCTTTACTGCAGTTTGATATTGAGTGTCTGTACCACATGTACAGG8130.13006168731442613No Hit
CATGGGAGAACGTCGTGAGACAGTTCGGTCCCTATCCGTCGCGGGCGTAGG8030.12846191256271117No Hit
ATCGCTCAACGGATAAAAGCTACCCTGGGGATAACAGGCTTATCTCCCCCA7940.12702211528616772No Hit
GCAGTTTGACTGGGGCGGTCGCCTCCTAAAAGGTAACGGAGGCGCCCAAAG7920.1267021603358247No Hit
CCAGCACCGGGCAGGCGTCACCCCCTATACATCATCTTACGATTTAGCAGA7920.1267021603358247No Hit
GCTGTAGTCGGTCCCAAGGGTTGGGCTGTTCGCCCATTAAAGCGGCACGCG7880.12606225043513875No Hit
CTGGTACACCAGCGGTAAGTCCACTCTGGTCCTCTCGTACTAGGAGCAGAT7800.12478243063376677No Hit
GCGACAGGACGGAAAGACCCCATGGAGCTTTACTGCAGTTTGATATTGAGT7740.1238225657827378No Hit
AACCCTTGGGACCGACTACAGCCCCAGGATGCGACGAGCCGACATCGAGGT7680.1228627009317088No Hit
CCTACATAGCTACCCAGCGATGCCTTTGGCAAGACAACTGGTACACCAGCG7650.12238276850619431No Hit
GGAAATTTGAGAGGATCTGCTCCTAGTACGAGAGGACCAGAGTGGACTTAC7310.11694353435036348No Hit
TCGTACTAGGAGCAGATCCTCTCAAATTTCCTACGCCCGCGACGGATAGGG7260.11614364697450599No Hit
GATCACTAAGCCCGACTTTCGTCCCTGCTCGAGTTGTAGCTCTCGCAGTCA7190.11502380464830553No Hit
GGTGCTGGAAGGTTAAGAGGAGTGCTTAGCGTAAGCGAAGGTATGAATTGA7080.11326405242141906No Hit
GCTCGAGTTGTAGCTCTCGCAGTCAAGCTCCCTTATACCTTTACACTCTGC7000.1119842326200471No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6990.1118242551448756No Hit
CCTCGATGTCGGCTCGTCGCATCCTGGGGCTGTAGTCGGTCCCAAGGGTTG6730.10766484079041672No Hit
ACTCTGGTCCTCTCGTACTAGGAGCAGATCCTCTCAAATTTCCTACGCCCG6700.10718490836490223No Hit
TCCATACGGAACCACCGGATCACTAAGCCCGACTTTCGTCCCTGCTCGAGT6650.10638502098904473No Hit
GACTTACCGCTGGTGTACCAGTTGTCTTGCCAAAGGCATCGCTGGGTAGCT6610.10574511108835875No Hit
ACCTTAGGACCGTTATTGTTACGGCCGCCGTTTACTGGGGCTTCAATTCAT6610.10574511108835875No Hit
CCATTCTGAGGGAACCTTTGGGCGCCTCCGTTACCTTTTAGGAGGCGACCG6530.10446529128698678No Hit
GGTGTACCAGTTGTCTTGCCAAAGGCATCGCTGGGTAGCTATGTAGGGAAG6510.1041453363366438No Hit
GTCAAACTGCCCGTCAGACACTGTCTCCGATAGGGATCACCTATCCGGGTT6460.10334544896078632No Hit
GCCGTTTACTGGGGCTTCAATTCATACCTTCGCTTACGCTAAGCACTCCTC6430.10286551653527183No Hit
GTGTTATGGCCACTCTAACCCGGATAGGTGATCCCTATCGGAGACAGTGTC6380.10206562915941436No Hit
CCATACGGAACCACCGGATCACTAAGCCCGACTTTCGTCCCTGCTCGAGTT6370.10190565168424286No Hit
GAGTACATGGGAGAACGTCGTGAGACAGTTCGGTCCCTATCCGTCGCGGGC6360.10174567420907137No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)