FastQCFastQC Report
Thu 15 Dec 2022
EGAF00007544273

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007544273
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences156355
Sequences flagged as poor quality0
Sequence length51
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34042.1770969908221676No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17061.0911067762463624No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7150.4572926993060663No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5310.33961178088324645No Hit
CTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTGAA4370.27949218125419717RNA PCR Primer, Index 31 (96% over 29bp)
GAGTACATGGGGTCTTTCCGTCTTTCCGCGGGGAGATTGCATCATCACAAA4000.25582808352786923No Hit
CTTTCCGCGGGGAGATTGCATCATCACAAACATTTCAACTTCGCTGAGTCT3560.22768699433980366No Hit
GCATGAACGGGTTGCAAAAAATCGGTGGCTGCGACTGTTTATTAAAAACAC2840.1816379393047872No Hit
TATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTT2730.17460266700777077RNA PCR Primer, Index 31 (95% over 22bp)
CACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGC2670.17076524575485275RNA PCR Primer, Index 38 (96% over 25bp)
GGTATCAACGCAGAGTACATGGGGTCTTTCCGTCTTTCCGCGGGGAGATTG2060.13175146301685267No Hit
CATCTTGAGGTGGGTTTCACACTTAGATGCTTTCAGCGTTTATCCCTTCCC1610.10297080361996737No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)